pBG167 Recombinant Sindbis Virus Expression Plasmid
Recombinant plasmid pBG167 expresses infectious Sindbis virus (SINV) and allows for fast and easy expression of your gene of interest.
Highlights:
- Plasmid can be transfected into mammalian cells to produce infectious Sindbis virus
- Unique XbaI site behind 3’ subgenomic promoter can be used to express genes of interest during infection
The entire Sindbis virus genome was inserted into the pcDNA3.1 Zeo+ expression vector such that the transcription start site of the CMV promoter overlaps with the first viral nucleotide of the viral genome. A Hepatitis D virus (HDV) ribozyme was added to the 3' end of the viral genomic 35bp polyA sequence. A unique XbaI restriction site 3' subgenomic promoter to allow genes of interest to be ligated into the viral genome that will be expressed only when the virus genome is replicated. Transfection of this construct into mammalian cells produces an infectious self-replicating Sindbis virus genome that can be modified to express genes of interest in vitro or in vivo.
From the laboratory of Brian J. Geiss, PhD, Colorado State University.
Recombinant plasmid pBG167 expresses infectious Sindbis virus (SINV) and allows for fast and easy expression of your gene of interest.
Highlights:
- Plasmid can be transfected into mammalian cells to produce infectious Sindbis virus
- Unique XbaI site behind 3’ subgenomic promoter can be used to express genes of interest during infection
The entire Sindbis virus genome was inserted into the pcDNA3.1 Zeo+ expression vector such that the transcription start site of the CMV promoter overlaps with the first viral nucleotide of the viral genome. A Hepatitis D virus (HDV) ribozyme was added to the 3' end of the viral genomic 35bp polyA sequence. A unique XbaI restriction site 3' subgenomic promoter to allow genes of interest to be ligated into the viral genome that will be expressed only when the virus genome is replicated. Transfection of this construct into mammalian cells produces an infectious self-replicating Sindbis virus genome that can be modified to express genes of interest in vitro or in vivo.
From the laboratory of Brian J. Geiss, PhD, Colorado State University.
| Product Type: | Plasmid |
| Gene/insert name: | Sindbis virus replicon |
| Organism: | Sindbis Virus Strain TE3'2J (AR339) |
| Antibiotic Resistance: | Amp |
| Grow in E. coli at 37 C: | DH5-alpha or XL10-Gold E-coli |
| Cloning Site 5': | MluI |
| Cloning Site 3': | XhoI |
| Insert Size: | 12,169 bp |
| Vector Backbone and Size: | pcDNA3.1 Zeo+ |
| High or low copy: | High |
| Storage: | Room Temperature (Dried), -20C (liquid) |
| Shipped: | Room Temperature |
- Geiss BJ, Shimonkevitz LH, Sackal CI, Olson KE. Recombination-ready Sindbis replicon expression vectors for transgene expression. Virol J. 2007 Oct 26;4:112.PubMed PMID: 17963504; PubMed Central PMCID: PMC2164957.
If you publish research with this product, please let us know so we can cite your paper.
