Actin Knock Out (Cre-Lox) MEF Cell Lines

Mouse embryonic fibroblast (MEF) cell lines with floxed Actb (beta-actin), Actg1 (gamma-actin), or Actb/Actg1 alleles. Gene knock-out (KO) under exogenous expression of Cre recombinase via Cre-Lox recombination.

Highlights:

  • Actb LL-Large T - Actb KO under exogenous Cre expression
  • Actg LL-Large T - Actg1 KO under exogenous Cre expression
  • Actb LL-Actg1 LL-Large T - Actb/Actg1 double KO under exogenous Cre expression

Beta-actin is a highly conserved protein involved in cell motility, structure, and integrity. Beta-actin, is one of the six isoforms in humans, and one of the two non-muscle cytoskeletal actins. Similar to beta-actin, gamma actin is widely expressed in cellular cytoskeletons of many tissues; in adult striated muscle cells, gamma-actin is localized to Z-discs and costamere structures, which are responsible for force transduction and transmission in muscle cells.

From the laboratory of James M. Ervasti, PhD, University Minnesota, Twin Cities.

Catalog Number Product Size AVAILABILITY Price Qty
EMN019-FP
Floxed Beta-Actin (Actb) KO MEF Cell Line (Actb LL-Large T)
1 vial In stock
Price: $499.00
EMN021-FP
Floxed Gamma-Actin (Actg1) KO MEF Cell Line (Actg1 LL-Large T)
1 vial In stock
Price: $499.00
EMN020-FP
Floxed Beta-Actin (Actb)/Gamma-Actin (Actg1) Double KO MEF Cell Line (Actb LL Actg1 LL-Large T)
1 vial In stock
Price: $499.00

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Specifications
Product Type: Cell Line
Cell Type: Embryonic fibroblast
Organism: Mouse
Source: Day 13.5 mouse embryos
Morphology: Fibroblastic
Biosafety Level: BSL1
Subculturing: Fast growing immortalized mouse embryonic fibroblasts, 1:10 Split
Growth Conditions: DMEM, 10% FBS, 1% Pen Strep, 0.5ug/mL Fungizone
Cryopreservation: Media + 5% DMSO
Comments: Cre-Lox recombination gene knock-out requires exogenous Cre expression. See: Patrinostro X, et al. Mol Biol Cell. 2017 Mar 15;28(6):771-782.
Storage: LN2
Shipped: Dry Ice
Provider
From the laboratory of James M. Ervasti, PhD, University Minnesota, Twin Cities.
References
  1. Patrinostro X, O'Rourke AR, Chamberlain CM, Moriarity BS, Perrin BJ, Ervasti JM. Relative importance of β(cyto)- and γ(cyto)-actin in primary mouse embryonic fibroblasts. Mol Biol Cell. 2017 Mar 15;28(6):771-782.

If you publish research with this product, please let us know so we can cite your paper.

 
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