Actin Knock Out (Cre-Lox) MEF Cell Lines
Mouse embryonic fibroblast (MEF) cell lines with floxed Actb (beta-actin), Actg1 (gamma-actin), or Actb/Actg1 alleles. Gene knock-out (KO) under exogenous expression of Cre recombinase via Cre-Lox recombination.
Highlights:
- Actb LL-Large T - Actb KO under exogenous Cre expression
- Actg LL-Large T - Actg1 KO under exogenous Cre expression
- Actb LL-Actg1 LL-Large T - Actb/Actg1 double KO under exogenous Cre expression
Beta-actin is a highly conserved protein involved in cell motility, structure, and integrity. Beta-actin, is one of the six isoforms in humans, and one of the two non-muscle cytoskeletal actins. Similar to beta-actin, gamma actin is widely expressed in cellular cytoskeletons of many tissues; in adult striated muscle cells, gamma-actin is localized to Z-discs and costamere structures, which are responsible for force transduction and transmission in muscle cells.
From the laboratory of James M. Ervasti, PhD, University Minnesota, Twin Cities.
Mouse embryonic fibroblast (MEF) cell lines with floxed Actb (beta-actin), Actg1 (gamma-actin), or Actb/Actg1 alleles. Gene knock-out (KO) under exogenous expression of Cre recombinase via Cre-Lox recombination.
Highlights:
- Actb LL-Large T - Actb KO under exogenous Cre expression
- Actg LL-Large T - Actg1 KO under exogenous Cre expression
- Actb LL-Actg1 LL-Large T - Actb/Actg1 double KO under exogenous Cre expression
Beta-actin is a highly conserved protein involved in cell motility, structure, and integrity. Beta-actin, is one of the six isoforms in humans, and one of the two non-muscle cytoskeletal actins. Similar to beta-actin, gamma actin is widely expressed in cellular cytoskeletons of many tissues; in adult striated muscle cells, gamma-actin is localized to Z-discs and costamere structures, which are responsible for force transduction and transmission in muscle cells.
From the laboratory of James M. Ervasti, PhD, University Minnesota, Twin Cities.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
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| Product Type: | Cell Line |
| Cell Type: | Embryonic fibroblast |
| Accession ID: | P60709 |
| Organism: | Mouse |
| Source: | Day 13.5 mouse embryos |
| Morphology: | Fibroblastic |
| Biosafety Level: | BSL1 |
| Subculturing: | Fast growing immortalized mouse embryonic fibroblasts, 1:10 Split |
| Growth Conditions: | DMEM, 10% FBS, 1% Pen Strep, 0.5ug/mL Fungizone |
| Cryopreservation: | Media + 5% DMSO |
| Comments: | Cre-Lox recombination gene knock-out requires exogenous Cre expression. See: Patrinostro X, et al. Mol Biol Cell. 2017 Mar 15;28(6):771-782. |
| Storage: | LN2 |
| Shipped: | Dry Ice |
- Patrinostro X, O'Rourke AR, Chamberlain CM, Moriarity BS, Perrin BJ, Ervasti JM. Relative importance of β(cyto)- and γ(cyto)-actin in primary mouse embryonic fibroblasts. Mol Biol Cell. 2017 Mar 15;28(6):771-782.
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