β2-glycoprotein I Antibodies

These murine monoclonal antibodies recognize β2-glycoprotein I.

Highlights:

  • 21C5-48.2 recognizes human β2-glycoprotein I alone or when bound to anionic phospholipid (e.g., cardiolipin), as well as bovine β2-glycoprotein I bound to anionic phospholipid. This antibody could serve as a positive control in an anti-β2-glycoprotein I or an anti-cardiolipin ELISA.
  • 12A1-A17.3 recognizes human β2-glycoprotein I alone or when bound to anionic phospholipid (e.g., cardiolipin). This antibody could serve as a positive control in an anti-β2-glycoprotein I ELISA. It is negative in an anti-cardiolipin ELISA in which bovine β2-glycoprotein I (e.g., fetal bovine serum) is the sole source of β2-glycoprotein I present, as the antibody does not recognize phospholipid-bound bovine β2-glycoprotein I. However, it should be positive in an anti-cardiolipin assay in which human β2-glycoprotein I is provided.

β2-glycoprotein I is a phospholipid-binding protein found in the blood and is known to bind to anionic phospholipids. Antibodies to β2-glycoprotein I are considered to be a subset of a larger group of antibodies called "anti-phospholipid antibodies". Anti-phospholipid antibodies recognize phospholipid-binding proteins (like β2-glycoprotein I), rather than phospholipids directly, and are clinically relevant antibodies in a clinical syndrome called "anti-phospholipid syndrome" (APS) and in systemic lupus erythematosus (SLE). They are associated with thrombotic events and pregnancy morbidity in these patients. Thus, our monoclonal anti-β2-glycoprotein I antibodies could be useful as positive controls in clinical assays that measure these antibodies (i.e., "anti-cardiolipin assays" [which detect antibodies that recognize bovine β2-glycoprotein I that is bound to cardiolipin] and "anti-β2-glycoprotein I assays" [which detect antibodies that recognize human β2-glycoprotein I].

From the laboratory of Joyce Rauch, PhD, McGill University.

Catalog Number Product Size AVAILABILITY Price Qty
EMG016
Anti-β2-Glycoprotein I [12A1-A17.3] Antibody, 100ug
100ug 3-5 weeks
Price: $299.00
EMG017
Anti-β2-Glycoprotein I [21C5-48.2] Antibody, 100ug
100ug 3-5 weeks
Price: $299.00
Specifications
Product Type: Antibody
Antigen: β2-glycoprotein I
Isotype: IgG1
Clonality: Monoclonal
Clone Name: 12A1-A17.3
21C5-48.2
Species Immunized: Mouse
Purification Method: Protein G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Storage: -20C
Shipped: Cold Packs
Provider
From the laboratory of Joyce Rauch, PhD, McGill University.

References
  1. Price, B.E., Rauch, J., Shia, M.A., Walsh, M.T., Lieberthal, W., Gilligan, H.M., O'Laughlin, T., Koh, J.S., and Levine, J.S. Antiphospholipid autoantibodies bind to apoptotic, but not viable, thymocytes in a β2-glycoprotein I-dependent manner. J. Immunol. 157: 2201-2208, 1996 (PMID: 8757347)
  2. Subang, R., Levine, J.S., Janoff, A.S., Davidson, S.M.K., Taraschi, T.F., Koike, T., Minchey, S.R., Whiteside, M., Tannenbaum, M., and Rauch, J. Phospholipid-bound β2-glycoprotein I induces the production of anti-phospholipid antibodies. J. Autoimmunity 15: 21-32, 2000 (PMID: 10936025).
  3. Laplante, P., Fuentes, R., Salem, D., Subang, R., Gillis, M.-A., Hachem, A., Farhat, N., Qureshi, S.T., Fletcher, C.A., Roubey, R.A.S., Merhi, Y., Thorin, É., Levine, J.S., Mackman, N., and Rauch, J. Anti-phospholipid antibody-mediated effects in an arterial model of thrombosis are dependent on toll-like receptor 4. Lupus 25, 162-176, 2016 (PMID: 26391610).

If you publish research with this product, please let us know so we can cite your paper.

 
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