β2-glycoprotein I Antibodies
These murine monoclonal antibodies recognize β2-glycoprotein I.
Highlights:
- 21C5-48.2 recognizes human β2-glycoprotein I alone or when bound to anionic phospholipid (e.g., cardiolipin), as well as bovine β2-glycoprotein I bound to anionic phospholipid. This antibody could serve as a positive control in an anti-β2-glycoprotein I or an anti-cardiolipin ELISA.
- 12A1-A17.3 recognizes human β2-glycoprotein I alone or when bound to anionic phospholipid (e.g., cardiolipin). This antibody could serve as a positive control in an anti-β2-glycoprotein I ELISA. It is negative in an anti-cardiolipin ELISA in which bovine β2-glycoprotein I (e.g., fetal bovine serum) is the sole source of β2-glycoprotein I present, as the antibody does not recognize phospholipid-bound bovine β2-glycoprotein I. However, it should be positive in an anti-cardiolipin assay in which human β2-glycoprotein I is provided.
β2-glycoprotein I is a phospholipid-binding protein found in the blood and is known to bind to anionic phospholipids. Antibodies to β2-glycoprotein I are considered to be a subset of a larger group of antibodies called "anti-phospholipid antibodies". Anti-phospholipid antibodies recognize phospholipid-binding proteins (like β2-glycoprotein I), rather than phospholipids directly, and are clinically relevant antibodies in a clinical syndrome called "anti-phospholipid syndrome" (APS) and in systemic lupus erythematosus (SLE). They are associated with thrombotic events and pregnancy morbidity in these patients. Thus, our monoclonal anti-β2-glycoprotein I antibodies could be useful as positive controls in clinical assays that measure these antibodies (i.e., "anti-cardiolipin assays" [which detect antibodies that recognize bovine β2-glycoprotein I that is bound to cardiolipin] and "anti-β2-glycoprotein I assays" [which detect antibodies that recognize human β2-glycoprotein I].
From the laboratory of Joyce Rauch, PhD, McGill University.
These murine monoclonal antibodies recognize β2-glycoprotein I.
Highlights:
- 21C5-48.2 recognizes human β2-glycoprotein I alone or when bound to anionic phospholipid (e.g., cardiolipin), as well as bovine β2-glycoprotein I bound to anionic phospholipid. This antibody could serve as a positive control in an anti-β2-glycoprotein I or an anti-cardiolipin ELISA.
- 12A1-A17.3 recognizes human β2-glycoprotein I alone or when bound to anionic phospholipid (e.g., cardiolipin). This antibody could serve as a positive control in an anti-β2-glycoprotein I ELISA. It is negative in an anti-cardiolipin ELISA in which bovine β2-glycoprotein I (e.g., fetal bovine serum) is the sole source of β2-glycoprotein I present, as the antibody does not recognize phospholipid-bound bovine β2-glycoprotein I. However, it should be positive in an anti-cardiolipin assay in which human β2-glycoprotein I is provided.
β2-glycoprotein I is a phospholipid-binding protein found in the blood and is known to bind to anionic phospholipids. Antibodies to β2-glycoprotein I are considered to be a subset of a larger group of antibodies called "anti-phospholipid antibodies". Anti-phospholipid antibodies recognize phospholipid-binding proteins (like β2-glycoprotein I), rather than phospholipids directly, and are clinically relevant antibodies in a clinical syndrome called "anti-phospholipid syndrome" (APS) and in systemic lupus erythematosus (SLE). They are associated with thrombotic events and pregnancy morbidity in these patients. Thus, our monoclonal anti-β2-glycoprotein I antibodies could be useful as positive controls in clinical assays that measure these antibodies (i.e., "anti-cardiolipin assays" [which detect antibodies that recognize bovine β2-glycoprotein I that is bound to cardiolipin] and "anti-β2-glycoprotein I assays" [which detect antibodies that recognize human β2-glycoprotein I].
From the laboratory of Joyce Rauch, PhD, McGill University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Antibody |
| Antigen: | Beta 2-glycoprotein I |
| Accession ID: | P02749 |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: |
12A1-A17.3 21C5-48.2 |
| Species Immunized: | Mouse |
| Purification Method: | Protein G |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Storage: | -20C |
| Shipped: | Cold Packs |
- Price, B.E., Rauch, J., Shia, M.A., Walsh, M.T., Lieberthal, W., Gilligan, H.M., O'Laughlin, T., Koh, J.S., and Levine, J.S. Antiphospholipid autoantibodies bind to apoptotic, but not viable, thymocytes in a β2-glycoprotein I-dependent manner. J. Immunol. 157: 2201-2208, 1996 (PMID: 8757347)
- Subang, R., Levine, J.S., Janoff, A.S., Davidson, S.M.K., Taraschi, T.F., Koike, T., Minchey, S.R., Whiteside, M., Tannenbaum, M., and Rauch, J. Phospholipid-bound β2-glycoprotein I induces the production of anti-phospholipid antibodies. J. Autoimmunity 15: 21-32, 2000 (PMID: 10936025).
- Laplante, P., Fuentes, R., Salem, D., Subang, R., Gillis, M.-A., Hachem, A., Farhat, N., Qureshi, S.T., Fletcher, C.A., Roubey, R.A.S., Merhi, Y., Thorin, É., Levine, J.S., Mackman, N., and Rauch, J. Anti-phospholipid antibody-mediated effects in an arterial model of thrombosis are dependent on toll-like receptor 4. Lupus 25, 162-176, 2016 (PMID: 26391610).
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