143B rho-0#25 Cell Line

The 143B rho-0#25 cell line is a derivative of the 143B human osteosarcoma cells, which have lost their mitochondrial DNA (mtDNA) as a result of transient transfection with plasmid pMA4008 encoding HSV UL12.5M185 protein, which damages mtDNA by an unknown mechanism.

Highlights:

  • Useful as recipients in production of cytoplasmic hybrids (cybrids)

From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.

Catalog Number Product Size AVAILABILITY Price Qty
ESA106
143B rho-0#25 Cell Line
1 vial 3-5 weeks
Price: $399.00
Specifications
Product Type: Cell Line
Cell Type: Osteosarcoma
Organism: Human
Source: 143B cell line
Morphology: Epithelial
Biosafety Level: BSL 1
Subculturing: Split 1:4 every 3 days
Growth Conditions: DMEM/10% FBS/ 1 mM pyruvate, 50 µg/ml uridine
Cryopreservation: Media + 10% DMSO
Comments: Slow- Growing
Storage: LN2
Shipped: Dry Ice
Provider
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.

References
  1. Spadafora D, Kozhukhar N, Chouljenko VN, Kousoulas KG, Alexeyev MF. Methods for Efficient Elimination of Mitochondrial DNA from Cultured Cells. PLoS One. 2016 May 2;11(5):e0154684.

If you publish research with this product, please let us know so we can cite your paper.

 
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