4B6 Cell Line

This cell line is a derivative of the Cre4 cell line (Cat. ESA109). Cre4 cells were engineered for Tet-On expression with a retrovirus 2641 (Mol. Biol. Rep. 37 (2010) 1987-1991), which encodes a Tet-On Advanced transactivator, EGFP, and blasticidin resistance, thus producing a clone designated 4B6.

From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.

Catalog Number Product Size AVAILABILITY Price Qty
ESA102
4B6 Cell Line
1 vial 3-5 weeks
Price: $399.00
Specifications
Product Type: Cell Line
Cell Type: Embryonic fibroblasts
Organism: Mouse
Source: Mouse E13.5 Embryo
Morphology: Spindle-shaped at confluence
Biosafety Level: BSL 1
Subculturing: Split 1:4 to 1:8 every 3 days
Growth Conditions: DMEM/10% FBS+10 µg/ml blasticidin
Cryopreservation: Media + 10% DMSO
Comments: Fast Growing
Storage: LN2
Shipped: Dry Ice
Provider
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.

Comments
Cre-TM mice (JAX 004682, B6.Cg-Tg(CAG-cre/Esr1)5Amc/J) were intercrossed with Lig3LoxP mice (Nature 471(2011) 240-244) to provide tamoxifen-inducible cre expression driven from the actin promoter. Lig3Cre-TM mouse embryonic fibroblast (MEF) cell cultures were prepared from E13.5 embryonic mesenchyme. Cells were allowed to proliferate in T-25 tissue culture flasks at 37 °C in a humidified CO2-regulated (5%) incubator and were immortalized with a retroviral construct 3315, which encodes SV40 large T antigen (J. Biol. Chem. 288 (2013) 26594-26605) and selected depending upon their ability to form colonies. One clone was selected for its superior growth characteristics and designated Cre4. This clone was transduced with a retrovirus 2641 (Mol. Biol. Rep. 37 (2010) 1987-1991, which encodes a Tet-On Advanced transactivator, EGFP and blasticidin resistance), thus producing a clone designated 4B6. 4B6 is a Tet-On derivative of the Cre4.
References
  1. Shokolenko IN, Fayzulin RZ, Katyal S, McKinnon PJ, Wilson GL, Alexeyev MF. Mitochondrial DNA ligase is dispensable for the viability of cultured cells but essential for mtDNA maintenance. J Biol Chem. 2013 Sep 13;288(37):26594-605.

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