3T3 rho-0#8 Cell Line

3T3 rho-0#8 cell line is a derivative of the NIH 3T3 cells, which have lost their mitochondrial DNA (mtDNA) as a result of transient transfection with plasmid pMA4008 encoding HSV UL12.5M185 protein, which damages mtDNA by an unknown mechanism.

Highlights:

  • Useful as recipients in production of cytoplasmic hybrids (cybrids)

     

From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.

Catalog Number Product Size AVAILABILITY Price Qty
ESA101
3T3 rho-0#8 Cell Line
1 vial 3-5 weeks
Price: $399.00
Specifications
Product Type: Cell Line
Cell Type: Fibroblasts
Organism: Mouse
Source: Mouse Embryo
Morphology: Spindle-shaped at confluence
Biosafety Level: BSL 1
Subculturing: Split 1:4 every 3 days
Growth Conditions: DMEM/10% FBS/ 1 mM pyruvate, 50 µg/ml uridine
Cryopreservation: Media + 10% DMSO
Comments: Slow- Growing
Storage: LN2
Shipped: Dry Ice
Provider
From the laboratory of Mikhail F Alexeyev, PhD, University of South Alabama.

References
  1. Spadafora D, Kozhukhar N, Alexeyev MF. Presequence-Independent Mitochondrial Import of DNA Ligase Facilitates Establishment of Cell Lines with Reduced mtDNA Copy Number. PLoS One. 2016 Mar 31;11(3):e0152705.

If you publish research with this product, please let us know so we can cite your paper.

 
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