Yeast-Display Nanobody Library (NbLib)

Yeast surface display nanobody library (NbLib) for rapid discovery of conformationally selective nanobodies.

Highlights

  • Fully in vitro platform for nanobody discovery based on yeast surface display

Camelid single-domain antibody fragments (‘nanobodies’) provide the remarkable specificity of antibodies within a single 15-kDa immunoglobulin VHH domain. This unique feature has enabled applications ranging from use as biochemical tools to therapeutic agents. Nanobodies have emerged as especially useful tools in protein structural biology, facilitating studies of conformationally dynamic proteins such as G-protein-coupled receptors (GPCRs).

From the laboratory of Andrew C. Kruse, PhD, Harvard University.

Catalog Number Product Size AVAILABILITY Price Qty
EF0014-FP
Yeast-Display Nanobody Library (NbLib), 1 vial (>2.5x10^9 cells)
1 vial (>2.5x10^9 cells) In stock
Price: $500.00

The sale of this product is restricted to Nonprofit customers only.

Customers CANNOT use this material for industrially sponsored academic research. Customers wishing to utilize this product for industrially sponsored academic research must first Contact Us before ordering.

For profit customers, please Contact Us for more information.

Specifications
Product Type: Bacteria
Name: Yeast-Display Nanobody Library (NbLib)
Organism: S. cerevisiae
Strain: BJ5465
Growth Conditions: Yglc4.5 –Trp media, shake at 230 RPM, 30C. PDF Suggested Protocol
Cryopreservation: Yglc4.5 –Trp media + 10% DMSO
Amount: 1 vial (>2.5x10^9 cells)
Comments: Includes one vial of library and one vial of sample cells for users to perform trial runs of growing yeast cultures, should they wish).
Storage: -80C
Shipped: Dry ice
Provider
From the laboratory of Andrew C. Kruse, PhD, Harvard University.
References
  1. McMahon C, Baier AS, Pascolutti R, Wegrecki M, Zheng S, Ong JX, Erlandson SC, Hilger D, Rasmussen SGF, Ring AM, Manglik A, Kruse AC. Yeast surface display platform for rapid discovery of conformationally selective nanobodies. Nat Struct Mol Biol. 2018 Mar;25(3):289-296. doi: 10.1038/s41594-018-0028-6. Epub 2018 Feb 12.

If you publish research with this product, please let us know so we can cite your paper.

 
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