Anti-Nesprin-2 [K20-478] Antibody

This mouse monoclonal antibody was generated against protein and recognizes Nesprin-2.

Highlights:

  • Reacts with Nesprin-2
  • Suitable for Western Blot, Electron Micograph and Immunofluorescence applications

Nesprins (nuclear envelope spectrin repeat proteins) are a family of proteins that are found primarily in the outer nuclear membrane, as well as other subcellular compartments. Nesprins are part of the LINC complex (Linker of Nucleoskeleton and Cytoskeleton) which is a protein network that associates the nuclear envelope (the membrane surrounding the nucleus) to the cytoskeleton, outside the nucleus, and the nuclear lamina, inside the nucleus.

From the laboratory of Ludwig Eichinger, PhD, University of Cologne.

Catalog Number Product Size AVAILABILITY Price Qty
ECO001
Anti-Nesprin-2 [K20-478] Antibody, 100ug
100ug 3-5 weeks
Price: $299.00
Specifications
Product Type: Antibody
Antigen: Nesprin-2
Clonality: Monoclonal
Clone Name: K20-478
Immunogen: Protein - DNA fragments encoding the first 285 (6´His-ABD) and 459 amino acid residues (6´His-ABD-l) were obtained by PCR using fragment 2 as a template and inserted into pQE-30 (Qiagen). Recombinant proteins were induced by IPTG in E. coli M15[pREP4] and purified using a Ni-NTA agarose column according to the manufacturer (Qiagen). mAb K20-478 was produced by immunizing mice with the purified 6´His-ABD-l protein with ImmunEasy mouse adjuvant (Qiagen). Zhen et al. 2002
Species Immunized: Mouse
Purification Method: Protein A/G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Storage: -20C
Shipped: Cold Packs
Provider
From the laboratory of Ludwig Eichinger, PhD, University of Cologne.

References
  1. Zhen YY, Libotte T, Munck M, Noegel AA, Korenbaum E. NUANCE, a giant protein connecting the nucleus and actin cytoskeleton. J Cell Sci. 2002 Aug 1;115(Pt 15):3207-22. PubMed PMID: 12118075.
  2. Libotte T, Zaim H, Abraham S, Padmakumar VC, Schneider M, Lu W, Munck M, Hutchison C, Wehnert M, Fahrenkrog B, Sauder U, Aebi U, Noegel AA, Karakesisoglou I. Lamin A/C-dependent localization of Nesprin-2, a giant scaffolder at the nuclear envelope. Mol Biol Cell. 2005 Jul;16(7):3411-24. Epub 2005 Apr 20. PubMed PMID: 15843432; PubMed Central PMCID: PMC1165422.
  3. Neumann S, Schneider M, Daugherty RL, Gottardi CJ, Eming SA, Beijer A, Noegel AA, Karakesisoglou I. Nesprin-2 interacts with {alpha}-catenin and regulates Wnt signaling at the nuclear envelope. J Biol Chem. 2010 Nov 5;285(45):34932-8.

If you publish research with this product, please let us know so we can cite your paper.

 
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