Serratia marcescens DNA/RNA Nonspecific Nuclease Expression Plasmid

Plasmid expressing Serratia marcescens DNA/RNA Nonspecific Nuclease for purification of recombinant proteins.

Highlights:

  • Reduces or eliminates nucleic acids from the disrupted cells
  • Greatly decreases the viscosity of the resultant solution

Serratia marcescens nuclease is a well known non-specific nuclease that digests single and double stranded RNA and DNA. It is widely used in the purification of recombinant proteins because it reduces or eliminates nucleic acids from the disrupted cells, and greatly decreases the viscosity of the resultant solution. This makes subsequent purification procedures easier. Commercial versions of this enzyme are available but expensive.

From the laboratory of Darrell L. Peterson, PhD, Virginia Commonwealth University.

Catalog Number Product Size AVAILABILITY Price Qty
EVC009
Serratia marcescens DNA/RNA Nonspecific Nuclease Expression Plasmid
Liquid (plasmid solution) In stock
Price: $66.00
Specifications
Product Type: Plasmid
Gene/insert name: DNA/RNA nonspecific nuclease
Organism: Serratia marcescens
Antibiotic Resistance: Ampicillin
Fusion Tag(s): OmpA signal, carboxyterminal histidine tag, Tev cleavage site
Grow in E. coli at 37 C: Yes
Selectable markers: Ampicillin, G418
Cloning Site 5': NdeI
Cloning Site 3': BamH1
Insert Size: 909 bp
Vector Backbone and Size: pET3
High or low copy: High
Storage: -20C
Shipped: Room Temperature
Provider
From the laboratory of Darrell L. Peterson, PhD, Virginia Commonwealth University.

References
  1. Friedhoff P, Gimadutdinow O, Rüter T, Wende W, Urbanke C, Thole H, Pingoud A. A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli. Protein Expr Purif. 1994 Feb;5(1):37-43. PubMed PMID: 8167472.
  2. Chen P, Yang H, Li H, Yang L, Li X. [Expression, purification and characterization of non-specific Serratia nuclease in Escherichia coli]. Sheng Wu Gong Cheng Xue Bao. 2011 Aug;27(8):1247-57. Chinese. PubMed PMID: 22097815.

If you publish research with this product, please let us know so we can cite your paper.

 
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