Serratia marcescens DNA/RNA Nonspecific Nuclease Expression Plasmid
Plasmid expressing Serratia marcescens DNA/RNA Nonspecific Nuclease for purification of recombinant proteins.
Highlights:
- Reduces or eliminates nucleic acids from the disrupted cells
- Greatly decreases the viscosity of the resultant solution
Serratia marcescens nuclease is a well known non-specific nuclease that digests single and double stranded RNA and DNA. It is widely used in the purification of recombinant proteins because it reduces or eliminates nucleic acids from the disrupted cells, and greatly decreases the viscosity of the resultant solution. This makes subsequent purification procedures easier. Commercial versions of this enzyme are available but expensive.
From the laboratory of Darrell L. Peterson, PhD, Virginia Commonwealth University.
Plasmid expressing Serratia marcescens DNA/RNA Nonspecific Nuclease for purification of recombinant proteins.
Highlights:
- Reduces or eliminates nucleic acids from the disrupted cells
- Greatly decreases the viscosity of the resultant solution
Serratia marcescens nuclease is a well known non-specific nuclease that digests single and double stranded RNA and DNA. It is widely used in the purification of recombinant proteins because it reduces or eliminates nucleic acids from the disrupted cells, and greatly decreases the viscosity of the resultant solution. This makes subsequent purification procedures easier. Commercial versions of this enzyme are available but expensive.
From the laboratory of Darrell L. Peterson, PhD, Virginia Commonwealth University.
Specifications
| Product Type: | Plasmid |
| Gene/insert name: | DNA/RNA nonspecific nuclease |
| Organism: | Serratia marcescens |
| Antibiotic Resistance: | Ampicillin |
| Fusion Tag(s): | OmpA signal, carboxyterminal histidine tag, Tev cleavage site |
| Grow in E. coli at 37 C: | Yes |
| Selectable markers: | Ampicillin, G418 |
| Cloning Site 5': | NdeI |
| Cloning Site 3': | BamH1 |
| Insert Size: | 909 bp |
| Vector Backbone and Size: | pET3 |
| High or low copy: | High |
| Storage: | -20C |
| Shipped: | Room Temperature |
Documentation
Provider
From the laboratory of Darrell L. Peterson, PhD, Virginia Commonwealth University.
References
- Friedhoff P, Gimadutdinow O, Rüter T, Wende W, Urbanke C, Thole H, Pingoud A. A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli. Protein Expr Purif. 1994 Feb;5(1):37-43. PubMed PMID: 8167472.
- Chen P, Yang H, Li H, Yang L, Li X. [Expression, purification and characterization of non-specific Serratia nuclease in Escherichia coli]. Sheng Wu Gong Cheng Xue Bao. 2011 Aug;27(8):1247-57. Chinese. PubMed PMID: 22097815.
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