Salutaridine Synthase (CYP719B1)

Salutaridine synthase CYP719B1 [EC:1.14.21.4] produced in Sf9 insect cells is a cytochrome P450 enzyme from Papaver somniferum that is able to convert the substrate (R)-reticuline to salutaridine. This enzyme functions in the morphine biosynthetic pathway.

From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.

Catalog Number Product Size AVAILABILITY Price Qty
EDD005
Salutaridine Synthase (CYP719B1), 350uL
350uL 1-2 weeks
Price: $250.00
Specifications
Product Type: Protein
Accession ID: EF451150
EC Number: 1.14.21.4
Source: Sf9 Cells
Molecular Weight: 56,498 Da
Buffer: 100 mM tricine pH 7.5/ 5 mM thioglycolic acid
Comments: This enzyme requires the addition of NADPH for catalysis. Produced in Sf9 insect cells in conjunction with cytochrome P450 reductase from Eschscholzia californica and 2 mg/l hemin. Sf9 cells are permeabilized by the storage buffer
Storage: -80C
Shipped: Dry Ice
Provider
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
References
  1. Gesell, A., M. Rolf, J. Ziegler, M. L. Diaz Chavez, F. C. Huang and T. M. Kutchan (2009). "CYP719B1 is salutaridine synthase, the C-C phenol-coupling enzyme of morphine biosynthesis in opium poppy." J Biol Chem 284(36): 24432-24442.

If you publish research with this product, please let us know so we can cite your paper.

 
Loading...
Loading...