Salutaridine Synthase (CYP719B1)
Salutaridine synthase CYP719B1 [EC:1.14.21.4] produced in Sf9 insect cells is a cytochrome P450 enzyme from Papaver somniferum that is able to convert the substrate (R)-reticuline to salutaridine. This enzyme functions in the morphine biosynthetic pathway.
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
Salutaridine synthase CYP719B1 [EC:1.14.21.4] produced in Sf9 insect cells is a cytochrome P450 enzyme from Papaver somniferum that is able to convert the substrate (R)-reticuline to salutaridine. This enzyme functions in the morphine biosynthetic pathway.
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
Specifications
| Product Type: | Protein |
| Accession ID: | EF451150 |
| EC Number: | 1.14.21.4 |
| Source: | Sf9 Cells |
| Molecular Weight: | 56,498 Da |
| Buffer: | 100 mM tricine pH 7.5/ 5 mM thioglycolic acid |
| Comments: | This enzyme requires the addition of NADPH for catalysis. Produced in Sf9 insect cells in conjunction with cytochrome P450 reductase from Eschscholzia californica and 2 mg/l hemin. Sf9 cells are permeabilized by the storage buffer |
| Storage: | -80C |
| Shipped: | Dry Ice |
Provider
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
References
- Gesell, A., M. Rolf, J. Ziegler, M. L. Diaz Chavez, F. C. Huang and T. M. Kutchan (2009). "CYP719B1 is salutaridine synthase, the C-C phenol-coupling enzyme of morphine biosynthesis in opium poppy." J Biol Chem 284(36): 24432-24442.
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