Cheilanthifoline Synthase (CYP719A14)

Cheilanthifoline synthase (CYP719A14) from Argemone mexicana produced in Sf9 insect cells is a P450 enzyme that catalyzes a methylenedioxy bridge on the molecule (S)-scoulerine to form (S)-cheilanthifoline, an essential step in the formation of benzo[c]phenanthridine and protoberberine alkaloids.

From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.

Catalog Number Product Size AVAILABILITY Price Qty
EDD007
Cheilanthifoline Synthase (CYP719A14), 350uL
350uL 1-2 weeks
Price: $250.00
Specifications
Product Type: Protein
Accession ID: EF451152
EC Number: 1.14.21.2
Source: Sf9 Cells
Molecular Weight: 55,898 Da
Buffer: 100 mM tricine pH 7.5/ 5 mM thioglycolic acid
Comments: This enzyme requires the addition of NADPH for catalysis. Produced in Sf9 insect cells in conjunction with cytochrome P450 reductase from Eschscholzia californica and 2 mg/l hemin. Sf9 cells are permeabilized by the storage buffer and are able to be used directly in enzyme assays.
Storage: -80C
Shipped: Dry Ice
Provider
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
References
  1. Díaz Chávez ML, Rolf M, Gesell A, Kutchan TM. Characterization of two methylenedioxy bridge-forming cytochrome P450-dependent enzymes of alkaloid formation in the Mexican prickly poppy Argemone mexicana. Arch Biochem Biophys. 2011 Mar 1;507(1):186-93. doi: 10.1016/j.abb.2010.11.016. Epub 2010 Nov 20. PubMed PMID: 21094631.

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