Cheilanthifoline Synthase (CYP719A14)
Cheilanthifoline synthase (CYP719A14) from Argemone mexicana produced in Sf9 insect cells is a P450 enzyme that catalyzes a methylenedioxy bridge on the molecule (S)-scoulerine to form (S)-cheilanthifoline, an essential step in the formation of benzo[c]phenanthridine and protoberberine alkaloids.
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
Cheilanthifoline synthase (CYP719A14) from Argemone mexicana produced in Sf9 insect cells is a P450 enzyme that catalyzes a methylenedioxy bridge on the molecule (S)-scoulerine to form (S)-cheilanthifoline, an essential step in the formation of benzo[c]phenanthridine and protoberberine alkaloids.
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
Specifications
| Product Type: | Protein |
| EC Number: | 1.14.21.2 |
| Accession ID: | B1NF20, EF451152 |
| Source: | Sf9 Cells |
| Molecular Weight: | 55,898 Da |
| Buffer: | 100 mM tricine pH 7.5/ 5 mM thioglycolic acid |
| Comments: | This enzyme requires the addition of NADPH for catalysis. Produced in Sf9 insect cells in conjunction with cytochrome P450 reductase from Eschscholzia californica and 2 mg/l hemin. Sf9 cells are permeabilized by the storage buffer and are able to be used directly in enzyme assays. |
| Storage: | -80C |
| Shipped: | Dry Ice |
Provider
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
References
- Diaz Chavez ML, Rolf M, Gesell A, Kutchan TM. Characterization of two methylenedioxy bridge-forming cytochrome P450-dependent enzymes of alkaloid formation in the Mexican prickly poppy Argemone mexicana. Arch Biochem Biophys. 2011 Mar 1;507(1):186-93. doi: 10.1016/j.abb.2010.11.016. Epub 2010 Nov 20. PubMed PMID: 21094631.
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