Anti-Lamin A+C [LASS2D9] Antibody

This mouse IgG3 kappa monoclonal antibody recognizes human and mouse Lamin A+C.

Highlights:

  • Reacts with human and mouse lamin A+C
  • Suitable for Western Blot, Immunocytochemistry, Immunohistochemistry and Immunofluorscence applications

Lamins A and C are major components of the nuclear lamina, a thin protein meshwork located at the nuclear face of the nuclear envelope (NE). The lamina provides structural integrity to the NE and is involved in many other aspects of nuclear biology including transcription and chromatin organization. Lamins A and C arise from the LMNA gene by alternative splicing and majority of adult tissues express at least one of the isoform. The LMNA gene has been extensively studies due to its association with variety of human diseases. Mutations in LMNA have been linked 12 distinct disorders, including Emery-Dreifus muscular dystrophy, dilated cardiomyopathy, Dunnigan-type partial lipodystrophy and Hutchinson-Gilford progeria syndrome.

From the laboratory of Brian Burke, PhD, A*STAR Institute of Medical Biology (IMB).

Catalog Number Product Size AVAILABILITY Price Qty
ESG012
Anti-Lamin A+C [LASS2D9] Antibody, 5mL (supernatant)
5mL supernatant 3-5 weeks
Price: $250.00
Specifications
Product Type: Antibody
Antigen: Lamin A+C
Accession ID: 4000
Molecular Weight: Lamin A 74kDa, Lamin C 65 kDa
Isotype: IgG3 kappa
Clonality: Monoclonal
Clone Name: LASS2D9
Reactivity: Human and Mouse
Species Immunized: Mouse
Buffer: Cell culture supernatant
Tested Applications: WB, IF, IHC, ICC
Storage: 80C
Shipped: Dry Ice
Data

Western Blot

Western blot analysis of LASS2D9 antibody. Whole cell lysate from human (Hela) and mouse (C2C12, mouse adult fibroblasts (MAFs)) cell lines cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and blotted with LASS2D9 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. The predicted molecular weight for lamin A is 74 kDA and for lamin C 65 kDa. The absence of a band in LMNA null MAFs indicated that the antibody specifically recognizes products of LMNA gene.

References
  1. Burke, B., C.L Stewart. 2013. The nuclear lamins: flexibility in function. Nat Rev Mol Cell Biol. 14(1):13-24.
  2. Xie, W., A. Chojnowski, T. Boudier, J. S. Lim, S. Ahmed, Z. Ser, C. Stewart, B. Burke. 2016. Atype Lamins Form Distinct Filamentous Networks with Differential Nuclear Pore Complex Associations. Curr Biol. 2016 Sep 9. pii: S0960-9822(16)30842-9. doi: 10.1016/j.cub.2016.07.049.

If you publish research with this product, please let us know so we can cite your paper.

 
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