Zebrafish Novel Immune-Type Receptor 9 (Nitr9) Antibodies

These IgG2, k light chain mouse monoclonal antibodies were raised against protein and recognize zebrafish novel immune-type receptor 9 (Nitr9).

Highlights:

  • Reacts with zebrafish novel immune-type receptor 9 (Nitr9)
  • Suitable for Immunofluorescence, Western Blot and Flow Cytometry applications

The novel immune-type receptor (NITR) family includes activating and inhibitory forms that are predicted to function as natural killer receptors in teleost species. The only activating NITR gene in zebrafish is nitr9. The zebrafish nitr9 gene yields three alternatively spliced transcripts that encode three proteins isoforms: Nitr9-long (Nitr9L), Nitr9-short (Nitr9S), and Nitr9-supershort (Nitr9SS). Nitr9 transcripts are detectable in immune tissues, but are present at significantly higher levels in lymphocytes than in myeloid cells. The Nitr9L protein has been shown to physically partner with and signals via the adaptor protein Dap12.

From the laboratory of Jeffrey A. Yoder, PhD, North Carolina State University.

Catalog Number Product Size AVAILABILITY Price Qty
EWP001
Anti-Zebrafish Novel Immune-Type Receptor 9 (Nitr9) [19.1.1] Antibody, 100ug
100ug 3-5 weeks
Price: $299.00
EWP002
Anti-Zebrafish Novel Immune-Type Receptor 9 (Nitr9) [90.10.5] Antibody, 100ug
100ug 3-5 weeks
Price: $299.00
Specifications
Product Type: Antibody
Accession ID: GenBank AY570237.1, AY570238.1, EF690658.1
Antigen: Zebrafish novel immune-type receptor 9 (Nitr9)
Molecular Weight: 34 kDa (Nitr9L), 30 kDa (Nitr9S) and 20 kDa (Nitr9SS). Nitr9L and Nitr9S may appear larger in western analyses due to glycosylation
Isotype: 19.1.1: IgG2a, k light chain
90.10.5: IgG2b, k light chain
Clonality: Monoclonal
Clone Name: 19.1.1
90.10.5
Reactivity: Zebrafish
Immunogen: Protein
Species Immunized: Mouse
Epitope: I-type Immunoglobulin domain (encoded by nucleotides 298-623 of GenBank NM 001005576.1)
Purification Method: Protein G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: 19.1.1: IF and FC with transfected cells (may not recognize Nitr9S isoform by FC)
90.10.5: WB with endogenous tissue (1:1000), IF and FC with transfected cells (may not recognize Nitr9S isoform by FC)
Comments: May not recognize Nitr9S isoform by FC
Storage: -20C
Shipped: Cold Packs
Provider
From the laboratory of Jeffrey A. Yoder, PhD, North Carolina State University.

References
  1. Shah RN, Rodriguez-Nunez I, Eason DD, Haire RN, Bertrand JY, Wittamer V, Traver D, Nordone SK, Litman GW, Yoder JA. 2012. Development and characterization of anti-Nitr9 antibodies. Adv Hematol.2012:596925. PMID: 2304955
  2. Muire PJ, Hanson LA, Wills R, Petrie-Hanson L. Differential gene expression following TLR stimulation in rag1-/- mutant zebrafish tissues and morphological descriptions of lymphocyte-like cell populations. PLoS One. 2017 Sep 14;12(9):e0184077. doi: 10.1371/journal.pone.0184077. eCollection 2017. PubMed PMID: 28910320

If you publish research with this product, please let us know so we can cite your paper.

 
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