Erythromycin A Production Plasmids

Plasmids for the expression of erythromycin A using E coli.

Erythromycin A is a potent antibiotic long-recognized as a therapeutic option for bacterial infections. The soil-dwelling bacterium Saccharopolyspora erythraea natively produces erythromycin A from a 55 kb gene cluster composed of three large polyketide synthase genes (each ~10 kb) and 17 additional genes responsible for deoxysugar biosynthesis, macrolide tailoring, and resistance.

From the laboratory of Blaine A. Pfeifer, PhD, Tufts University.

Catalog Number Product Size AVAILABILITY Price Qty
ETU006
pHZT1 Plasmid, 150ng
Spotted on filter paper In stock
Price: $66.00
ETU007
pHZT2 Plasmid, 150ng
Spotted on filter paper In stock
Price: $66.00
Specifications
Product Type: Plasmid
Gene/insert name: pHZT1, pHZT2
Antibiotic Resistance: pHZT1: Kan
pHZT2: Carb
Fusion Tag(s): pHZT1: eryBI,BII,BIII,BIV,BV,BVI,BVII,ermE
pHZT2: eryCI,CII,CIII,CIV,CV,CVI,eryF,eryG,eryK
Grow in E. coli at 37 C: Yes
Cloning Site 5': XbaI
Cloning Site 3': SpeI
Insert Size: 13.2 kb
Vector Backbone and Size: pHZT1: pET28a, 5369 bp
pHZT2: pET21c, 5441 bp
High or low copy: Low
Storage: -20C
Shipped: Room Temperature
Provider
From the laboratory of Blaine A. Pfeifer, PhD, Tufts University.
Comments

Adapted from: Zhang, Haoran, et al. Chemistry & biology 17.11 (2010): 1232-1240.

References
  1. Zhang, Haoran, et al. "Complete biosynthesis of erythromycin A and designed analogs using E. coli as a heterologous host." Chemistry & biology 17.11 (2010): 1232-1240.

If you publish research with this product, please let us know so we can cite your paper.

 
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