13C6-UV Cleavable Biotin-Azide

This isotopically labeled “heavy” tag can be used in parallel to the unlabeled “light” analog (See: UV Cleavable Biotin-Azide) to screen protein adduction by identifying hits as those with a mass difference of 6 Da.

Highlights:

  • Biotin conjugate can be subsequently visualized by streptavidin Western techniques and/or enriched using streptavidin purification methods
  • Captured target can be released under mild photolysis conditions (365 nm) and results in a significant decrease of background signal due to non-specifically bound proteins

From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.

Catalog Number Product Size AVAILABILITY Price Qty
EVU151
13C6-UV Cleavable Biotin-Azide, 3mg
3mg In stock
Price: $750.00
Specifications
Product Type: Small Molecule
Name: 13C6-UV-Biotin-azide
Chemical Formula: C3213C6H51N7O9S
Molecular Weight: 787.88
Format: Pale yellow powder
Purity: >95%
Solubility: DMSO, MeOH
Spectral Information: 1H, 13C NMR
Comments: Protect from light and hydrolysis conditions
Storage: Upon receipt store at -20C
Shipped: Ambient temperature
Data
ClickReaction
Provider
From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
References
  1. Quantitative Chemoproteomics for Site-Specific Analysis of Protein Alkylation by 4-Hydroxy-2-Nonenal in Cells. Yang, J.; Tallman, K. A.; Porter, N.A.; Liebler, D. C. Analytical Chemistry 2015, 87(5), 2535.
  2. A Chemoproteomic Platform to Assess Bioactivation Potential of Drugs. Sun, R.; Shi, F.; Liu, K.; Fu, L.; Tian, C.; Yang, Yong; Tallman, K. A.; Porter, N. A.; Yang, J. Chemical Research in Toxicology 2017, 30(10), 1797.

If you publish research with this product, please let us know so we can cite your paper.

 
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