Anti-Centromere Protein F (CENP-F) Antibodies

These IgG mouse monoclonal antibodies were produced against mouse c-terminal centromere binding protein F (CENP-F) and recognize human, mouse, dog and quail CENP-F.

Highlights:

  • Reacts with human, mouse, dog and quail CENP-F in cell lines derived from these organisms
  • Characterization studies show that all antibodies react with transfected CENP-F expressed in COS cells in Western Blot and immunoflourescence assays.
  • Antibodies react with endogenous CENP-F using immunoflourescence assays using cultured cells derived from the species listed above
  • Suitable for Immunofluorescence applications

C-terminal domain of centromere binding protein F associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. Shown to be involved in the regulation of DNA synthesis, cell cycle progression. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. Examination of the literature on CENP-F reveals great complexity in function, expression, and cellular localization in different cell types and different species.

From the laboratory of David M. Bader, PhD, Vanderbilt University.

Catalog Number Product Size AVAILABILITY Price Qty
EVU146
Anti-CENP-F [REMELDA 9] Antibody, 5mL (supernatant)
5mL Supernatant 3-5 weeks
Price: $225.00
EVU147
Anti-CENP-F [REMELDA 16] Antibody, 5mL (supernatant)
5mL Supernatant 3-5 weeks
Price: $225.00
EVU148
Anti-CENP-F [REMELDA 17] Antibody, 5mL (supernatant)
5mL Supernatant 3-5 weeks
Price: $225.00
EVU149
Anti-CENP-F [REMELDA 18] Antibody, 5mL (supernatant)
5mL Supernatant 3-5 weeks
Price: $225.00
EVU150
Anti-CENP-F [REMELDA 30] Antibody, 5mL (supernatant)
5mL Supernatant 3-5 weeks
Price: $225.00
Specifications
Product Type: Antibody
Antigen: Centromere Binding Protein F (CENP-F)
Molecular Weight: 347-380 kDa
Isotype: IgG
Clonality: Monoclonal
Clone Name: REMELDA 9
REMELDA 16
REMELDA 17
REMELDA 18
REMELDA 30
Reactivity: Human, mouse, dog and quail
Immunogen: Mouse C-terminal CENP-F
Species Immunized: Mouse
Buffer: Cell culture supernatant
Tested Applications: IF
Comments: This monoclonal antibody works at high specificity with little background when used in hybridoma medium. Purification is not needed for standard immunochemical analyses.
Storage: -80C
Shipped: Dry Ice
Provider
From the laboratory of David M. Bader, PhD, Vanderbilt University.
Comments

Note: Variation in subcellular protein localization may be observed using diverse cell types from different organisms.

Clone Hela HCT MDCK QM7
Remelda 9 Mitotic Spindle Mitotic Spindle - Mitotic Spindle
Remelda 16 Peripheral cytoplasmic domain Broad cytoplasm Perinuclear/small nuclear puncta Perinuclear/small nuclear puncta
Remelda 17 Peripheral cytoplasmic domain/ Large nuclear puncta Large nuclear puncta Cytoplasmic strands Small nuclear puncta/peripheral cytoplasmic domain
Remelda 18 Perinuclear Perinuclear Small nuclear puncta Small nuclear puncta
Remelda 30 Large nuclear puncta Large nuclear puncta Large nuclear puncta Cytoplasm
References
  1. Reid W. McCullough, Emily L. Mace, Elise R. Pfaltzgraff, and David M. Bader. Monoclonal Antibodies against Centromere Binding Protein F. In Review.

If you publish research with this product, please let us know so we can cite your paper.

 
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