6dEB Plasmids

These plasmids (pBP144 and pBP130, used together) allow for the production of 6-deoxyerythronolide B (6dEB), the polyketide precursor to erythromycin, when residing in a strain of E. coli (such as BAP1) capable of supporting heterologous polyketide production.

From the laboratories of Blaine A. Pfeifer, PhD, University at Buffalo and Chaitan Khosla, PhD, Stanford University.

Catalog Number Product Size AVAILABILITY Price Qty
ESU003
pBP130-6dEB Plasmid
Liquid plasmid In stock
Price: $66.00
ESU004
pBP144-6dEB Plasmid
Liquid plasmid In stock
Price: $66.00
Specifications
Product Type: Plasmid
Gene/insert name: pBP130, pBP144
Fusion Tag(s): pbBP130: DEBS2, DEBS3
pbBP144: PCC, DEBS1
Antibiotic Resistance: pbBP130: Ampicillin
pbBP144: Kanamycin
Grow in E. coli at 37 C: Yes
Selectable markers: pbBP130: Ampicillin
pbBP144: Kanamycin
Vector Backbone and Size: pbBP130: pET21c, 5443 bp
pbBP144: pET28a, 5369 bp
High or low copy: Medium
Comments: Please see the reference below and associated supplementary information for cloning details and generalized plasmid maps.
Storage: -20C
Shipped: Room Temperature
Provider
From the laboratories of Blaine A. Pfeifer, PhD, University at Buffalo and Chaitan Khosla, PhD, Stanford University.
References
  1. Pfeifer BA, Admiraal SJ, Gramajo H, Cane DE, Khosla C. Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli. Science. 2001; 291(5509):1790-2.

If you publish research with this product, please let us know so we can cite your paper.

Related Products
 
Loading...
Loading...