Anti-CD6 [34-3] Antibody

This rat IgG2b monoclonal antibody was raised against full length CDNA, whole CD6-transfected NB2 cells and is specific for mouse T-cell differentiation antigen CD6.

Highlights:

  • Reacts with mouse CD6
  • Binds to the extracellular domain of CD6
  • Suitable for Flow Cytometry applications

T-cell differentiation antigen CD6 is a cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses. It has been shown to interact with ALCAM/CD166. Cd6 is found on the outer membrane of T-lymphocytes as well as some other immune cells. CD6 contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. It is important protein for continuation of T cell activation.

Catalog Number Product Size AVAILABILITY Price Qty
ESU001
Anti-CD6 [34-3] Antibody, 100ug
100ug 3-5 weeks
Price: $299.00
Specifications
Product Type: Antibody
Accession ID: Q61003
Antigen: mouse CD6
Molecular Weight: 130 kDa
Isotype: IgG2b
Clonality: Monoclonal
Clone Name: 34-3
Reactivity: Mouse
Immunogen: Protein
Species Immunized: Rat
Epitope: Extracellular domain
Purification Method: Protein A/G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: Flow Cytometry (1 µg detects CD6 in one million mouse splenocytes)
Storage: -20C
Shipped: Cold Packs
Data

Tissue distribution of mouse CD6 detected with anti-mCD6 mAb #34-3.

Panel A shows anti-CD6 co-staining CD3+ (top row) and CD5+ (bottom row) lymphocytes from peripheral blood, lymph node, spleen and thymus. Panel B shows CD6 expression on CD4+ (top row) or CD8+ (bottom row) T cells from peripheral blood, lymph node, spleen and thymus.

Comments
Spleen cells were then fused by standard methods with the mouse myeloma line, Ag8.653, and plated out in 96-well plates in complete DMEM supplemented with HEPES, 20% fetal calf serum, 20% thymocyte conditioned medium, and HAT (hypoxanthine, aminopterine, thymidine) (Sigma, St. Louis, MO). Supernatants from wells that were positive for growth under HAT selection were screened by flow cytometry for anti-CD6 reactivity against the CD6-transfected NB2 rat T cell line. Clone #34 was found to be IgG2b. mAb #34 was subcloned once again and the subclone designated #34-3 was used for subsequent studies.
References

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