Monosomy 3 Uveal Melanoma Cell Lines

Cell lines UPMM-1 and UPMM-2 are derived from different donors with primary uveal melanoma tumors with monosomy 3.

Highlights:

  • Cells are positive for melanoma markers HMB45, S100 and MCSP
  • UPMM-1 -Monosomy 3; GNAQ, R183 mutation (retains the chromosome 3 status of the primary tumor)
  • UPMM-2 -Monosomy 3; GNAQ, Q209 mutation (chromosome 3 has undergone duplication (isodisomy) and is present on the background of a hypotetraploid karyotype)

Uveal melanoma (UM) is a cancer (melanoma) of the eye involving the iris, ciliary body, or choroid (collectively referred to as the uvea). Tumors arise from the pigment cells (melanocytes) that reside within the uvea giving color to the eye. Primary UM can be devided in two major classes. Metastatic progression is typically seen in one class of UM characterized by monosomy 3, but is rare in tumors with disomy 3. Therefore, monosomy 3 cell lines are most suitable for analyses of mechanisms and pathways contributing to metastatic progression. There is a tremendous market need, because currently only one alternative cell line is available with monosomy 3.

From the laboratory of Michael Zeschnigk, PhD, University of Duisburg-Essen.

Catalog Number Product Size AVAILABILITY Price Qty
EDE001
Uveal Melanoma Cell Line (UPMM-1)
1 vial In stock
Price: $599.00
EDE002
Uveal Melanoma Cell Line (UPMM-2)
1 vial Currently unavailable
Price: $599.00
Specifications
Product Type: Cell Line
Organism: Human
Accession ID: UPMM-1: CVCL_C299
Subculturing: Split 1x a week
Growth Conditions: Ham's F12 with 10-20% FCS, 100 IU/ml penicillin, 100ug/ml streptomycin, CO2 5%, O2 20%
Source: Uveal Melanoma
Donor Attributes: UPMM-1 amd UPMM-2 are from different donors (both are from primary tumors with monosomy 3 in male caucasian subjects)
Comments: Karyotyping performed via conventional GTG-banding. See: Nareyeck G, et al. Exp Eye Res. 2006 Oct;83(4):858-64.
Storage: LN2
Shipped: Dry Ice
Provider
From the laboratory of Michael Zeschnigk, PhD, University of Duisburg-Essen.
References
  1. Nareyeck G, Zeschnigk M, Prescher G, Lohmann DR, Anastassiou G. Establishment and characterization of two uveal melanoma cell lines derived from tumors with loss of one chromosome 3. Exp Eye Res. 2006 Oct;83(4):858-64. Epub 2006 Jun 5. Review. PubMed PMID: 16750193.

If you publish research with this product, please let us know so we can cite your paper.

 
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