Zika Virus Plasmids
pJW231 and pJW232 plasmids offer an infections clone system that produce virus with in vitro and in vivo characteristics similar to those of the primary isolate, providing a critical tool to study ZIKV infection and disease.
Highlights:
- Two-plasmid infectious clone system was created, from which infectious virus was rescued that replicates in human and mosquito cells with growth kinetics representative of wild-type ZIKV
- pJW231 (Plasmid 1) - Encodes ZIKV 5’ end (1-3498)
- pJW232 (Plasmid 2) - Encodes ZIKV 3’ end (3,109-10,809) and HDV Ribozyme
- Infectious clone-derived virus initiated infection and transmission rates in Aedes aegypti mosquitoes comparable to those of the primary isolate and displayed similar pathogenesis in AG129 mice
Zika virus (ZIKV; family Flaviviridae, genus Flavivirus) is a rapidly expanding global pathogen that has been associated with severe clinical manifestations, including devastating neurological disease in infants. This ZIKV reverse genetics system based on a 2015 isolate from Puerto Rico (strain PRVABC59). This infectious clone produces virus with in vitro and in vivo characteristics similar to those of the primary isolate, providing a critical tool to study ZIKV infection and disease.
From the laboratories of Brian J. Geiss, PhD and Gregory D. Ebel, ScD, Colorado State University.
pJW231 and pJW232 plasmids offer an infections clone system that produce virus with in vitro and in vivo characteristics similar to those of the primary isolate, providing a critical tool to study ZIKV infection and disease.
Highlights:
- Two-plasmid infectious clone system was created, from which infectious virus was rescued that replicates in human and mosquito cells with growth kinetics representative of wild-type ZIKV
- pJW231 (Plasmid 1) - Encodes ZIKV 5’ end (1-3498)
- pJW232 (Plasmid 2) - Encodes ZIKV 3’ end (3,109-10,809) and HDV Ribozyme
- Infectious clone-derived virus initiated infection and transmission rates in Aedes aegypti mosquitoes comparable to those of the primary isolate and displayed similar pathogenesis in AG129 mice
Zika virus (ZIKV; family Flaviviridae, genus Flavivirus) is a rapidly expanding global pathogen that has been associated with severe clinical manifestations, including devastating neurological disease in infants. This ZIKV reverse genetics system based on a 2015 isolate from Puerto Rico (strain PRVABC59). This infectious clone produces virus with in vitro and in vivo characteristics similar to those of the primary isolate, providing a critical tool to study ZIKV infection and disease.
From the laboratories of Brian J. Geiss, PhD and Gregory D. Ebel, ScD, Colorado State University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Plasmid |
| Gene/insert name: |
pJW231 (aka p1-ZIKV): T7 Promoter/Zika virus (strain PRVABC59) 5' end (1-3498) pJW232 (aka p2-ZIKV): Zika virus (strain PRVABC59) 3' end (3,109-10,809) and HDV Ribozyme |
| Antibiotic Resistance: | Ampicillin |
| Fusion Tag(s): | None |
| Grow in E. coli at 37 C: | DH5-alpha E. Coli or similar. Can grow at 37C. |
| Selectable markers: | None |
| Cloning Site 5': |
pJW231: ApaLI pJW232: SapI |
| Cloning Site 3': |
pJW231: PmlI pJW232: PvuI |
| Insert Size: |
pJW231: 3834 bp pJW232: 9458 bp |
| Vector Backbone and Size: | pACYC177 |
| High or low copy: | Low |
| Comments: | Using chloramphenicol amplification can increase plasmid yield. |
| Storage: | -20C |
| Shipped: | Room Temperature |
- Weger-Lucarelli J, Duggal NK, Bullard-Feibelman K, Veselinovic M, Romo H, Nguyen C, Rückert C, Brault AC, Bowen RA, Stenglein M, Geiss BJ, Ebel GD. Development and Characterization of Recombinant Virus Generated from a New World Zika Virus Infectious Clone. J Virol. 2016 Dec 16;91(1). pii: e01765-16. Print 2017 Jan 1. Erratum in: J Virol. 2017 Mar 29;91(8):. PubMed PMID: 27795432
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