Anti-Fli1b Antibody
This rabbit polyclonal antibody was generated against recombinant protein and is specific for zebrafish Fli1b protein (Fli1b).
Highlights:
- Reacts with zebrafish Fli1b
- Binds to the N-terminal half of Fli1b
- Suitable for Western Blot and Whole Mount Immunostaining applications
Fli1b protein is a transcription factor that is essential for vascular differentiation and morphogenesis in vertebrates.
From the laboratory of Nathan D. Lawson, PhD, University of Massachusetts Medical School.
This rabbit polyclonal antibody was generated against recombinant protein and is specific for zebrafish Fli1b protein (Fli1b).
Highlights:
- Reacts with zebrafish Fli1b
- Binds to the N-terminal half of Fli1b
- Suitable for Western Blot and Whole Mount Immunostaining applications
Fli1b protein is a transcription factor that is essential for vascular differentiation and morphogenesis in vertebrates.
From the laboratory of Nathan D. Lawson, PhD, University of Massachusetts Medical School.
| Product Type: | Antibody |
| Antigen: | Fli1b |
| Accession ID: | Q01543, NP_001008780 |
| Molecular Weight: | 51 kDa |
| Clonality: | Polyclonal |
| Reactivity: | Zebrafish |
| Immunogen: | Recombinant protein |
| Species Immunized: | Rabbit |
| Epitope: | N-terminal half of Fli1b |
| Buffer: | Polyclonal serum with 0.02% sodium azide |
| Tested Applications: | WB (1:5000), Whole mount immunostaining (1:500) |
| Comments: | Recommend aliquotting into small working volumes after initial thaw and store these at -80°C |
| Storage: | -80C |
| Shipped: | Cold Packs (Domestic, Overnight); Dry Ice (International) |
Immunohistochemistry
Upper panel: Whole mount immunohistochemistry, using 17-24 hours post fertilization wild type zebrafish embryos (2%PFA fixed). Primary stain: anti-Fl1b rabbit polyclonal antibody (1:500 dilution). Secondary stain: Invitrogen Goat anti-rabbit Alexa fluor 488 (Cat#:A111034); [1:1,000 Dilution].
Lower panel: Whole mount immunohistochemistry of 48 hours post fertilization zebrafish embryos, visualizing head (left) and trunk (right) regions. Primary stain: anti-Fli1b rabbit polyclonal antibody (1:500 dilution). Secondary stain: Invitrogen donkey anti-rabbit Alexa fluor 568 (Cat#:A10042); [1:1,000 Dilution].
- Moore JC, Sheppard-Tindell S, Shestopalov IA, Yamazoe S, Chen JK, Lawson ND. Post-transcriptional mechanisms contribute to Etv2 repression during vascular development. Dev Biol. 2013 Dec 1;384(1):128-40.
- Villefranc JA, Nicoli S, Bentley K, Jeltsch M, Zarkada G, Moore JC, Gerhardt H, Alitalo K, Lawson ND. A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development. Development. 2013 Apr;140(7):1497-506.
If you publish research with this product, please let us know so we can cite your paper.
