Anti-Ecdysteroid E/20E Antibodies and Antigens

Anti-Ecdysteroid E/20E Antibodies and Antigens

These rabbit polyclonal antibodies were generated against BSA conjugated ecdysteroids E and 20E and, in combination with BLG-conjugated ecdysteroids E and 20E, allow for a fast, accurate and low-cost means to measure titers for ecdysone (E) and 20-hydroxyecdysone (20E).

Highlights:

  • EAB25 is specific for 20-Hydroxyecdysone (20E) and should be purchased with the 20E-BLG conjugate, which is used to coat the assay plate
  • EAB27 recognizes both ecdysone (E) and 20-hydroxyecdysone (20E), and somewhat makisterone A and can be used with either E-BLG or 20E-BLG to coat plates
  • Neither antiserum recognizes 7-dehydrocholesterol
  • Ecdysone (E)-Bovine beta lactoglobulin (BLG) or 20-Hydroxyecdysone (20E)-BLG conjugates, for use with enzyme immunoassay
  • Suitable for enzyme immunoassay (See: Protocol); Not suitable for immunocytochemistry or western blots

Ecdysteroid hormones regulate several aspects of insect development and reproduction. The predominant ecdysteroids produced by insects including mosquitoes are ecdysone (E) and 20-hydroxyecdysone (20E). The ability to measure E and 20E titers is essential for many studies. These antibodies BLG-conjugated antigens allow for a fast, accurate and low-cost means to measures titers for ecdysone (E) and 20-hydroxyecdysone (20E).

From the laboratory of Mark R. Brown, PhD and Michael R. Strand, PhD, University of Georgia.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EGA262
Anti-Ecdysteroid 20E [EAB25] Antibody, 50uL
50uL 1-2 weeks
Regular Price:$345.00
On Sale:
EGA263
Anti-Ecdysteroid E/20E [EAB27] Antibody, 100uL
100uL In stock
Regular Price:$345.00
On Sale:
EGA264
20-Hydroxyecdysone (20E), BLG Conjugate, 100uL (0.4mg/mL)
100uL (0.4mg/mL) In stock
Regular Price:$120.00
On Sale:
EGA265
Ecdysone (E), BLG Conjugate, 100uL (0.4mg/mL)
100uL (0.4mg/mL) 1-2 weeks
Regular Price:$120.00
On Sale:
Specifications

Product Type: Antibody
Accession ID: P34021
Clonality: Polyclonal
Specificity: EAB25: specific for 20-Hydroxyecdysone (20E)
EAB27: recognizes both ecdysone (E) and 20-hydroxyecdysone (20E), and somewhat makisterone A
Species Immunized: Rabbit
Buffer: Whole serum for antibodies, PBS with 0.02% sodium azide for Ecdysteroid-BLG conjugates
Tested Applications: Enzymelinked immunoassay (EIA) (Protocol); Not suitable for immunocytochemistry or western blots
Comments: Antisera EAB25 and EAB27 were produced to ecdysone (E) conjugated to bovine serum albumin (BSA) or to 20-Hydroxyecdysone (20E) conjugated to BSA, so they should not be used in an enzyme immunoassay with BSA.
Storage: -80C
Shipped: Cold Packs (Domestic, Overnight); Dry Ice (International)

Documentation

PDF  Enzymelinked Immunoassay (EIA) Protocol

Data

Table of Antisera Properties

Antisera (antigen) Affinity Final use conc. Detection Range (pg)
EAB25 (20E-BSA) 20E 1:40,000 20E: 30-2,000
EAB27 (E-BSA) E, 20E 1:3,000 E/20E: 4-500

Adapted from: McKinney DA, et al. (2017) Gen Comp Endocrinol. 253:60-69.

Provider
From the laboratory of Mark R. Brown, PhD and Michael R. Strand, PhD, University of Georgia.
Comments
Antisera EAB25 and EAB27 were produced to ecdysone (E) conjugated to bovine serum albumin (BSA) or to 20-Hydroxyecdysone (20E) conjugated to BSA, so they should not be used in an enzyme immunoassay with BSA.
References
  1. McKinney DA, Strand MR, Brown MR. 2017 Evaluation of ecdysteroid antisera for a competitive enzyme immunoassay and extraction procedures for the measurement of mosquito ecdysteroids. Gen Comp Endocrinol. 253:60-69.
  2. Wang CF, Zhang Z, Sun W. Ecdysone oxidase and 3-dehydroecdysone-3β-reductase contribute to the synthesis of ecdysone during early embryonic development of the silkworm. Int J Biol Sci. 2018 Aug 6;14(11):1472-1482. View Article

If you publish research with this product, please let us know so we can cite your paper.