Anti-Pectic polysaccharide (beta-1,4-galactan) [LM5] Antibody
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This rat IgG monoclonal antibody was generated against polysaccharide and recognizes land plants pectic polysaccharide, beta-1,4-galactan.
Highlights:
- Reacts with land plants pectic polysaccharide, beta-1,4-galactan
- No cross-reactivity with (1-3)-Β-D-galactans or (1-6)-Β-D-galactans
- Suitable for Immunofluorescence and ELISA applications
The antibody was generated using a neoglycoprotein (galactotetraose-BSA). It recognizes a linear tetrasaccharide in (1-4)-Β-D-galactans. It has no cross-reactivity with (1-3)-Β-D-galactans or (1-6)-Β-D-galactans. It can recognize pectic polysaccharides in several species. In competitive inhibition ELISA, antibody binding to (1-4)-Β-D-galactan was inhibited (50%) by 58 μg/ml (1-4)-Β-D-galactotetraose and by 0.7 μg/ml lupin (1-4)-Β-D-galactan. Homogalacturonan is a pectic polysaccharide made up of α-1,4 linked galacturonic acid residues. Pectin is made up of a complex set of polysaccharides that are found in most primary cell walls and are particularly abundant in the non-woody parts of terrestrial plants. Pectin is a major component of the middle lamella, where it helps to bind cells together, but is also found in primary cell walls.
From the laboratory of Paul Knox, PhD, University of Leeds.
This rat IgG monoclonal antibody was generated against polysaccharide and recognizes land plants pectic polysaccharide, beta-1,4-galactan.
Highlights:
- Reacts with land plants pectic polysaccharide, beta-1,4-galactan
- No cross-reactivity with (1-3)-Β-D-galactans or (1-6)-Β-D-galactans
- Suitable for Immunofluorescence and ELISA applications
The antibody was generated using a neoglycoprotein (galactotetraose-BSA). It recognizes a linear tetrasaccharide in (1-4)-Β-D-galactans. It has no cross-reactivity with (1-3)-Β-D-galactans or (1-6)-Β-D-galactans. It can recognize pectic polysaccharides in several species. In competitive inhibition ELISA, antibody binding to (1-4)-Β-D-galactan was inhibited (50%) by 58 μg/ml (1-4)-Β-D-galactotetraose and by 0.7 μg/ml lupin (1-4)-Β-D-galactan. Homogalacturonan is a pectic polysaccharide made up of α-1,4 linked galacturonic acid residues. Pectin is made up of a complex set of polysaccharides that are found in most primary cell walls and are particularly abundant in the non-woody parts of terrestrial plants. Pectin is a major component of the middle lamella, where it helps to bind cells together, but is also found in primary cell walls.
From the laboratory of Paul Knox, PhD, University of Leeds.
| Product Type: | Antibody |
| Antigen: | Pectic polysaccharide (beta 1,4-galactan) |
| Isotype: | IgG |
| Clonality: | Monoclonal |
| Clone Name: | LM5 |
| Reactivity: | Land plants |
| Immunogen: | Polysaccharide |
| Species Immunized: | Rat |
| Epitope: | beta-1,4-galactan |
| Buffer: | Cell Culture Supernatant, 0.05% Sodium Azide |
| Tested Applications: | IF (1:10), ELISA (1:10) |
| Storage: | +4C (short term), -20C (long term) |
| Shipped: | Ambient temperature |
Immunofluorescence
Carrot Root Tip
- Jones L, Seymour GB, Knox JP. Localization of Pectic Galactan in Tomato Cell Walls Using a Monoclonal Antibody Specific to (1[->]4)-[beta]-D-Galactan. Plant Physiol. 1997 Apr;113(4):1405-1412.
- Andersen MC, Boos I, Marcus SE, Kra?un SK, Rydahl MG, Willats WG, Knox JP, Clausen MH. Characterization of the LM5 pectic galactan epitope with synthetic analogues of Β-1,4-d-galactotetraose. Carbohydr Res. 2016 Dec 21;436:36-40.
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