Anti-Pectic polysaccharide (Homogalacturonan) [LM7] Antibody

This rat IgM monoclonal antibody was generated against polysaccharide and recognizes land plants pectic polysaccharide, homogalacturonan.

Highlights:

  • Reacts with land plants pectic polysaccharide, homogalacturonan
  • Binds to paritally methyl esterified homogalacturonan
  • Does not bind to un-esterified homogalacturonan
  • Suitable for Immunofluorescence and ELISA applications

The antibody recognizes the homogalacturonan domain of pectic polysaccharides. It can recognize pectic polysaccharides in several species. The antibody recognizes a partially methyl-esterified epitope of HG that results from non-blockwise de-esterification processes. It does not bind to un-esterified homogalacturonan. Homogalacturonan is a pectic polysaccharide made up of α-1,4 linked galacturonic acid residues. Pectin is made up of a complex set of polysaccharides that are found in most primary cell walls and are particularly abundant in the non-woody parts of terrestrial plants. Pectin is a major component of the middle lamella, where it helps to bind cells together, but is also found in primary cell walls.

From the laboratory of Paul Knox, PhD, University of Leeds.

Catalog Number Product Size AVAILABILITY Price Qty
ELD006
Anti-Pectic polysaccharide (Homogalacturonan) [LM7] Antibody, 5mL (supernatant)
5mL 3-5 weeks
Price: $299.00
Specifications
Product Type: Antibody
Antigen: Pectic polysaccharide. Homogalacturonan
Isotype: IgM
Clonality: Monoclonal
Clone Name: LM7
Reactivity: Land plants
Immunogen: Polysaccharide
Species Immunized: Rat
Epitope: Partially methyl esterified homogalacturonan
Buffer: Cell Culture Supernatant, 0.05% Sodium Azide
Tested Applications: IF (1:10), ELISA (1:10)
Storage: +4C (short term), -20C (long term)
Shipped: Ambient temperature
Provider
From the laboratory of Paul Knox, PhD, University of Leeds.
References
  1. Willats WG, Orfila C, Limberg G, Buchholt HC, van Alebeek GJ, Voragen AG, Marcus SE, Christensen TM, Mikkelsen JD, Murray BS, Knox JP. Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls. Implications for pectin methyl esterase action, matrix properties, and cell adhesion. J Biol Chem. 2001 Jun 1;276(22):19404-13.
  2. Clausen MH, Willats WG, Knox JP. Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr Res. 2003 Aug 12;338(17):1797-800.

If you publish research with this product, please let us know so we can cite your paper.

 
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