Marek's Disease Kidney Cell Line (MDCC-UD35)
MDCC (Marek's disease cell culture) UD35 cells were established from a liver-localized lymphoma using the RB-1B strain of Marek's disease virus (MDV).
Marek's disease is a highly contagious viral neoplastic disease in chickens. Marek's disease is caused by an alphaherpesvirus known as 'Marek's disease virus' (MDV) or Gallid herpesvirus 2 (GaHV-2). The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes.
From the laboratory of Mark S. Parcells, PhD, University of Delaware.
MDCC (Marek's disease cell culture) UD35 cells were established from a liver-localized lymphoma using the RB-1B strain of Marek's disease virus (MDV).
Marek's disease is a highly contagious viral neoplastic disease in chickens. Marek's disease is caused by an alphaherpesvirus known as 'Marek's disease virus' (MDV) or Gallid herpesvirus 2 (GaHV-2). The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes.
From the laboratory of Mark S. Parcells, PhD, University of Delaware.
Specifications
| Product Type: | Cell Line |
| Cell Type: | Chicken T-cells (CD4hi, TCR2hi, CD3hi, CD28lo, CD29hi, CD30hi, CD44hi, CD45lo, CD164lo, MHC-Ihi, MHC-IIlo) |
| Source: | Liver lymphoma |
| Morphology: | non-adherent, pleomorphic, lymphoblastoid |
| Organism: | Chicken, Gallus gallus, (SPF single-comb white leghorn) |
| Biosafety Level: | BSL-1 |
| Subculturing: | 1:5 to 1:10 every 2 - 3 days |
| Growth Conditions: | Established using: Iscove's modified Dulbecco minimal essential medium (IMDMEM) supplemented with 20% FBS,10% chicken serum, 10% tryptose-phosphate broth, 4 mM L-glutamine, 2 µM 2-mercaptoethanol, 1X nonessential amino acids, 1X insulin / transferrin / selenium (ITS), 1X Pen/Strept/Neo, 1X fungizone. Adapted to: DMEM, high glucose, 10% FBS, 4 mM L-glutamine, 1 X PSN, 1X fungizone |
| Cryopreservation: | 90% FBS/10% DMSO |
| Comments: | Grow at 41C, 5% CO2. When actively growing, double every 12 -24 hrs. Grow at 5 x 105 to 2 x 107 cells/ml. |
| Storage: | Liquid nitrogen |
| Shipped: | Dry Ice |
Provider
From the laboratory of Mark S. Parcells, PhD, University of Delaware.
References
- Kumar, P, H Dong, D Lenihan, S Gaddamanugu, U Katneni, S Shaikh, P Tavlarides-Hontz, SM Reddy, W Peters, and MS Parcells. 2012. Selection of a recombinant Marek's disease virus in vivo through expression of the Marek's EcoRI-Q (Meq)-encoded oncoprotein: characterization of an rMd5-based mutant expressing the Meq of strain of RB-1B. Avian Dis. 56:328-340.
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