Sf9 Easy Titer Cell Line (Sf-9 ET)
Stably transfected Sf9 cells which emit eGFP upon infection with baculovirus.
Highlights
- Stably transfected with plasmid DNA containing the enhanced green fluorescent protein (eGFP) gene under the control of the baculovirus polyhedrin promoter
- Used in titration assay to determine baculovirus titer in lieu of CPE or Plaque assays
- 96-well plate format end-point dilution assay allows for titers to be determined in three days using a fluorescence microscope
The baculovirus expression vector system (BEVS) has proven to be a powerful technology for expression of recombinant proteins in eukaryotic cells. The BEVS technology has many advantages that include the capacity for large DNA insertions, the capacity for simultaneous expression of multiple genes, high expression levels for many proteins, and posttranslational modifications that often resemble those found in mammalian cells.
From the laboratory of Dominic Esposito, PhD, National Cancer Institute/NIH.
Stably transfected Sf9 cells which emit eGFP upon infection with baculovirus.
Highlights
- Stably transfected with plasmid DNA containing the enhanced green fluorescent protein (eGFP) gene under the control of the baculovirus polyhedrin promoter
- Used in titration assay to determine baculovirus titer in lieu of CPE or Plaque assays
- 96-well plate format end-point dilution assay allows for titers to be determined in three days using a fluorescence microscope
The baculovirus expression vector system (BEVS) has proven to be a powerful technology for expression of recombinant proteins in eukaryotic cells. The BEVS technology has many advantages that include the capacity for large DNA insertions, the capacity for simultaneous expression of multiple genes, high expression levels for many proteins, and posttranslational modifications that often resemble those found in mammalian cells.
From the laboratory of Dominic Esposito, PhD, National Cancer Institute/NIH.
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| Product Type: | Cell Line |
| Name: | Sf-9 ET |
| Alternative Name(s): | SF-9ET; Sf-9 Easy Titer; Sf9-ET; Sf9-Easy Titer; Sf9 Easy Titer |
| Cell Type: | Epithelial-like |
| Accession ID: | CVCL_1D71 |
| Source: | Ovary |
| Organism: | Spodoptera frugiperda |
| Morphology: | Rounded Sphere in suspension and when adhered |
| Biosafety Level: | 2 |
| Growth Conditions: | Sf-900 III SFM + 50 micrograms/ml Geneticin |
| Subculturing: | Seed at 1 X 106 cells/ml for 3-day transfer, 8 X 105 cells/ml for 4-day transfer |
| Cryopreservation: | 80% HyClone SFX, 10% FBS, 10% DMSO |
| Donor Attributes: | Female |
| Comments: | Transfected with: UniProtKB; P42212; GFP. |
| Storage: | Liquid nitrogen |
| Shipped: | Dry Ice |
- Gillette WK, Esposito D, Taylor TE, Hopkins RF, Bagni RK, Hartley JL. Purify First: rapid expression and purification of proteins from XMRV. Protein Expr Purif. 2011 Apr;76(2):238-47.
- Hopkins R, Esposito D. A rapid method for titrating baculovirus stocks using the Sf-9 Easy Titer cell line. Biotechniques. 2009 Sep;47(3):785-8.
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