Endoplasmic Reticulum Related Services
Offered Services:
- Analysis of all ER stress pathways and their modulation.
- Cytotoxicity and apoptosis assays.
- Protein subcellular localization and dynamics in fixed and live cells.
- Analysis of protein folding, glycosylation, turnover rate, trafficking.
Interested in one of the above services? Please contact us with your specific needs or any questions.
From the laboratory of Gerardo Z. Lederkremer, PhD, Tel Aviv University.
Offered Services:
- Analysis of all ER stress pathways and their modulation.
- Cytotoxicity and apoptosis assays.
- Protein subcellular localization and dynamics in fixed and live cells.
- Analysis of protein folding, glycosylation, turnover rate, trafficking.
Interested in one of the above services? Please contact us with your specific needs or any questions.
From the laboratory of Gerardo Z. Lederkremer, PhD, Tel Aviv University.
Specifications
| Product Type: | Service |
Documentation
Specific Research Specialties:
- Protein misfolding diseases, including neurodegenerative diseases.
- ER stress.
- Mechanisms of endoplasmic reticulum (ER) protein folding and quality control.
- Sugar chain trimming processes as cellular signals for glycoprotein folding status.
- Delivery of misfolded proteins to ER-associated degradation (ERAD).
- Subcellular compartmentalization of ER quality control and ERAD.
Research Methods:
- Biochemical and imaging studies in mammalian cells.
- Cytotoxicity and apoptosis assays.
- Subcellular fractionation, density gradients.
- Protein analysis: Western blot, immunoprecipitation, folding and glycosylation analysis. Protein interactions, coimmunoprecipitation.
- Radioactive pulse chase analysis.
- DNA cloning and engineering of constructs, mutants, fluorescent fusion proteins.
- RNA analysis, qPCR.
- Gene expression by cell transfection and infection, retrovirus and lentivirus vectors. Stable cell line selection.
- SiRNA and shRNA knockdown. Caspr/Cas9.
- Subcellular localization and dynamics. Immunofluorescence, confocal and live cell imaging. FRET, FRAP, FLIP.
Provider
From the laboratory of Gerardo Z. Lederkremer, PhD, Tel Aviv University.
References
If you publish research with this product, please let us know so we can cite your paper.
