Biotinylated Polyphosphate, Medium Chain (p100)

Biotinylated Polyphosphate, Medium Chain (p100)

This polyphosphate (Medium Chain, Biotinylated) is heterogeneous in size, with approximate polymer lengths ranging from ~45-160 phosphate units; modal size is about 75 phosphate units.

Highlights

  • Very similar size distribution to natural polyphosphates secreted by platelets
  • Contains biotin covalently attached to one or both of the terminal phosphates of the polyphosphate chain
  • Can be immobilized on streptavidin-coated multiwell plates or beads for binding studies of proteins to polyphosphate

Polyphosphate, a linear polymer of inorganic orthophosphate, which is widespread in living organisms and which plays diverse roles in biology, has recently been shown to be a potent modulator of the human blood clotting system. Its procoagulant activity is dependent on polymer length. This biotinylated, medium-chain polyphosphate (p100) is very similar in size distribution to natural polyphosphates secreted by platelets.

In addition to being a useful tool for plasma clotting assays, polyphosphate is can be used to study bacterial growth, virulence, and bacterial membrane function.

Also available:

From the laboratory of James H. Morrissey, PhD, University of Michigan.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EUI007
Biotinylated Polyphosphate, Medium Chain (p100)
120ug In stock
Regular Price:$230.00
On Sale:

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Specifications

Product Type: Small Molecule
Name: Polyphosphate, Medium Chain (p100; biotinylated)
Chemical Formula: (NaPO3)n[actual counterions may be Na &/or Li; nis approximately 45 to 160]
Molecular Weight: Heterogeneous; Number average ~11.5 kDa; Range ~2 kDa to 28.5 kDa
Format: Lyophilized
Purity: <1% monophosphate
Buffer: 42 mM Tris-HCl pH 8.5, 208 mM NaCl, 4.2 mM EDTA
Solubility: At least 100 mM in water; <1 mM in buffers containing divalent metals (make intermediate dilutions in divalent metal ion free buffers)
Concentration: When reconstituted:10 mM (phosphate monomer) NOTE: To estimate the polymer concentration, divide the phosphate monomer concentration by the modal polymer length
Size Distribution: Mode of n: 75 (as measured by migration on PAGE)
Comments: It is recommended to use ~10.2 ug of the biotin-polyphosphate to coat all wells of a streptavidin-coated 96-well plate.
Storage: Dry material should be stored at room temperature (or lower) with dessication.

Once reconstituted:
- Stable at least 12 hours at room temperature
- Stable at least 72 hours at 4C
- Store long-term at -80C
- Aliquot for repeated use, avoiding repeated freeze-thaw cycles
- Do not store in the presence of divalent metal ions
Shipped: Ambient temperature

Data

Size Distribution

Provider
From the laboratory of James H. Morrissey, PhD, University of Michigan.
Comments

Polyphosphate has limited solubility in the presence of divalent metal ions, so make intermediate dilutions in water, or in buffers without divalent metals or polyphosphate-binding proteins.

Reconstitution:
1 - Centrifuge tube 1 min at 1000 x g to pellet material in bottom of tube.
2 - Reconstitute in 118 µL purified water to make a solution of 10 mM phosphate.
NOTE: May be reconstituted in larger volumes to make a more dilute solution as needed.

References
  1. Morrissey JH, Choi SH, and Smith S.A. Polyphosphate: an ancient molecule that links platelets, coagulation and inflammation. Blood 119:5972-5979, 2012.
  2. Rao NN, Gómez-García MR, and Kornberg A. Inorganic polyphosphate: essential for growth and survival. Annu Rev Biochem. 78:605-647, 2009.
  3. Dinarvand P, Hassanian SM, Qureshi SH, Manithody C, Eissenberg JC, Yang L, Rezaie AR. Polyphosphate amplifies proinflammatory responses of nuclear proteins through interaction with receptor for advanced glycation end products and P2Y1 purinergic receptor. Blood. 2014 Feb 6;123(6):935-45.
  4. Suess PM, Gomer RH. Extracellular polyphosphate inhibits proliferation in anautocrine negative feedback loop in Dictyostelium discoideum. J Biol Chem. 2016 Aug 12. pii: jbc.M116.737825. View Article
  5. Morrissey JH. Poly-P as Modulator of Hemostasis, Thrombosis, and Inflammation. Blood 2017 130:SCI-1.
  6. Bentley-DeSousa A, Holinier C, Moteshareie H, Tseng YC, Kajjo S, Nwosu C, Amodeo GF, Bondy-Chorney E, Sai Y, Rudner A, Golshani A, Davey NE, Downey M. A Screen for Candidate Targets of Lysine Polyphosphorylation Uncovers a Conserved Network Implicated in Ribosome Biogenesis. Cell Rep. 2018 Mar 27;22(13):3427-3439. View Article
  7. Lorenzo-Orts L, Hohmann U, Zhu J, Hothorn M. Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules. Life Sci Alliance. 2019 May 27;2(3). pii: e201900385. View Article
  8. Suess PM, Chinea LE, Pilling D, Gomer RH. Extracellular Polyphosphate Promotes Macrophage and Fibrocyte Differentiation, Inhibits Leukocyte Proliferation, and Acts as a Chemotactic Agent for Neutrophils. J Immunol. 2019 Jul 15;203(2):493-499. View article
  9. Negreiros RS, Lander N, Huang G, Cordeiro CD, Smith SA, Morrissey JH, Docampo  R. Inorganic polyphosphate interacts with nucleolar and glycosomal proteins in trypanosomatids. Mol Microbiol. 2018 Dec;110(6):973-994.  View article 

If you publish research with this product, please let us know so we can cite your paper.