Anti-Mycoplasma gallisepticum Surface Epitope Antibodies
These IgG monoclonal antibodies were generated against various strains of Mycoplasma galliespticum and recognize M. gallisepticum surface epitopes.
Highlights:
- Reacts with M. gallisepticum surface epitopes
- Have the potential to be used in vaccine development, as additional components
- 12D10.H5 and 6A10.H6 exhibited similar inhibiting potency against 4 HA units of five different Mg strains. Partial HI was observed at Mab concentration from 20 to 50 µg/mL, while 4 to 16 times higher concentration yielded complete HI, but appear not to inhibit haemadsorption
- 3D6.C6 reacts strongly with p98 protein (98 kDa, PI=5.2) and with F, R, S6, A5969 and ts11 strains, and does not react with M. synoviae or M. iowae, and very little/no reaction is observed against 6/85 membrane
- 3D6.C6 has potential for serodiagnostic development
- Suitable for ELISA, DIBA, Immunoblotting, Immunoperoxidase assay and Haemagglutination Inhibition applications
Mycoplasma gallisepticum (Mg) is a bacterium belonging to the class Mollicutes and the family Mycoplasmataceae. It is the causative agent of chronic respiratory disease (CRD) in chickens and infectious sinusitis in turkeys, chickens, game birds, pigeons, and passerine birds, causing some levels of mortality. Once infected, they are carriers for the disease for life.Since the disease causes reduced feed and growth production, carcass condemnations, and retarded growth in juveniles, serious economic losses have occurred. Also, chickens have been documented to lose about 16 eggs over their laying cycle of 45 weeks. Mycoplasma lack cell walls, have highly variable surface proteins and a distinctive plasma membrane, and are the smallest self-replicating prokaryotes. Mycoplasma can cause disease in humans, animals, insects, and plants. Isolates of Mycoplasma gallisepticum (Mg) vary widely in their pathogenicity depending on the nature of the isolate, its method of propagation, and the number of passages. Among Mg strains significant differences have also been observed in their tissue tropism, transmission and in the immune response they induce.
From the laboratories of Stanley H. Kleven, DVM, PhD and Maricarmen García, PhD, University of Georgia.
These IgG monoclonal antibodies were generated against various strains of Mycoplasma galliespticum and recognize M. gallisepticum surface epitopes.
Highlights:
- Reacts with M. gallisepticum surface epitopes
- Have the potential to be used in vaccine development, as additional components
- 12D10.H5 and 6A10.H6 exhibited similar inhibiting potency against 4 HA units of five different Mg strains. Partial HI was observed at Mab concentration from 20 to 50 µg/mL, while 4 to 16 times higher concentration yielded complete HI, but appear not to inhibit haemadsorption
- 3D6.C6 reacts strongly with p98 protein (98 kDa, PI=5.2) and with F, R, S6, A5969 and ts11 strains, and does not react with M. synoviae or M. iowae, and very little/no reaction is observed against 6/85 membrane
- 3D6.C6 has potential for serodiagnostic development
- Suitable for ELISA, DIBA, Immunoblotting, Immunoperoxidase assay and Haemagglutination Inhibition applications
Mycoplasma gallisepticum (Mg) is a bacterium belonging to the class Mollicutes and the family Mycoplasmataceae. It is the causative agent of chronic respiratory disease (CRD) in chickens and infectious sinusitis in turkeys, chickens, game birds, pigeons, and passerine birds, causing some levels of mortality. Once infected, they are carriers for the disease for life.Since the disease causes reduced feed and growth production, carcass condemnations, and retarded growth in juveniles, serious economic losses have occurred. Also, chickens have been documented to lose about 16 eggs over their laying cycle of 45 weeks. Mycoplasma lack cell walls, have highly variable surface proteins and a distinctive plasma membrane, and are the smallest self-replicating prokaryotes. Mycoplasma can cause disease in humans, animals, insects, and plants. Isolates of Mycoplasma gallisepticum (Mg) vary widely in their pathogenicity depending on the nature of the isolate, its method of propagation, and the number of passages. Among Mg strains significant differences have also been observed in their tissue tropism, transmission and in the immune response they induce.
From the laboratories of Stanley H. Kleven, DVM, PhD and Maricarmen García, PhD, University of Georgia.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Antibody |
| Antigen: | Surface epitopes of M. gallisepticum |
| Molecular Weight: | 56-58 [and 79, strain dependent] |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | 8F7.A5 |
| Reactivity: | M. gallisepticum |
| Immunogen: | Strain F |
| Species Immunized: | Mouse |
| Purification Method: | Protein G |
| Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide |
| Tested Applications: | ELISA, DIBA, Immunoblotting, Immunoperoxidase assay and Haemagglutination inhibition |
| Storage: | -20C |
| Shipped: | Cold packs |
Mycoplasma gallisepticum Surface Epitope Hybridomas
| Cell Type: | Hybridoma |
| Designators: | Surface epitopes of M. gallisepticum |
| Media: | |
| Preservation: | 90% FBS, 10% DMSO |
| Storage Temperature: | Liquid Nitrogen |
| Shipped: | Dry Ice |
Clone Characteristics
Adapted from: Bencina D, et al. Avian Pathol. 1994 Mar;23(1):19-36.
- Bencina D, Kleven SH, Elfaki MG, Snoj A, Dovc P, Dorrer D, Russ I. Variable expression of epitopes on the surface of Mycoplasma gallisepticum demonstrated with monoclonal antibodies. Avian Pathol. 1994 Mar;23(1):19-36.
- García M, Elfaki MG, Kleven SH. Analysis of the variability in expression of Mycoplasma gallisepticum surface antigens. Vet Microbiol. 1994 Nov;42(2-3):147-58.
- Elfaki, MG, Kleven SH, Ragland, WL. Identification of specie-specific glycoprotein on the surface of Mycoplasma gallisepticum. FASEB. J., 5: A516.
- Avakian AP, Kleven SH, Ley DH. Comparison of Mycoplasma gallisepticum strains and identification of immunogenic integral membrane proteins with Triton X-114 by immunoblotting. Vet Microbiol. 1991 Nov;29(3-4):319-28.
- Elfaki MG, Kleven SH, Ragland WL, Steffens WL, Blankenship LL. Evidence for a common epitope on the surface of Mycoplasma gallisepticum defined by monoclonal antibody. Vet Microbiol. 1993 May;35(1-2):161-77.
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