Renal Proximal Tubule Cell Line (RPT-I8)
Immortalized renal proximal tubule (RPT) cell line derived from primary rabbit kidney proximal tubule cell cultures which were transfected with the pRSV-T vector containing SV40 early region genes.
Highlights:
- Immortalized – retain differentiated functions of RPT cells after many passages
- Display Na+-dependent a-MG uptake
- Express Brush border enzymes (γ-glutamyl transpeptidase and alkaline phosphatase)
- Respond to PTH by producing cAMP
The renal proximal tubule is part of the nephron (structural and functional unit of the kidney) containing the tubular fluid filtered through the glomerulus. The renal proximal tubule contains the components, proximal convulated tubule, loop of henle and the distal convulated tubule. Damage to the tubule can lead to kidney diseases such as Diabetes or Glomerular Disease.
From the laboratory of Mary L. Taub, PhD, University of Buffalo.
Immortalized renal proximal tubule (RPT) cell line derived from primary rabbit kidney proximal tubule cell cultures which were transfected with the pRSV-T vector containing SV40 early region genes.
Highlights:
- Immortalized – retain differentiated functions of RPT cells after many passages
- Display Na+-dependent a-MG uptake
- Express Brush border enzymes (γ-glutamyl transpeptidase and alkaline phosphatase)
- Respond to PTH by producing cAMP
The renal proximal tubule is part of the nephron (structural and functional unit of the kidney) containing the tubular fluid filtered through the glomerulus. The renal proximal tubule contains the components, proximal convulated tubule, loop of henle and the distal convulated tubule. Damage to the tubule can lead to kidney diseases such as Diabetes or Glomerular Disease.
From the laboratory of Mary L. Taub, PhD, University of Buffalo.
| Product Type: | Cell Line |
| Name: | RPT-I8 |
| Cell Type: | Renal Proximal Tubule |
| Accession ID: | CVCL_HA72 |
| Morphology: | Epithelial |
| Organism: | Rabbit |
| Source: | Kidney |
| Biosafety Level: | BSL2 |
| Subculturing: | Passage between 1/5 to 1/20 |
| Growth Conditions: | DME/F12 + 10% FCS also defined as Medium K-1 |
| Cryopreservation: | DME/F12 + 10% glycerol |
| Storage: | Liquid nitrogen |
| Shipped: | Dry ice |
Immunostaining
Expression of SV40 T antigen by RPT-I8 cells. Monolayers of RPT-I8 cells were fixed, and immunostained using either (A) a monoclonal anti-SV40 antibody or, as a control, (B) mouse monoclonalIgG2A.
Adapted from: Taub M, et al., Exp Cell Res. 2002 Dec 10;281(2):205-12.- Taub M, Han HJ, Rajkhowa T, Allen C, Park JH. Clonal analysis of immortalized renal proximal tubule cells: Na(+)/glucose cotransport system levels are maintained despite a decline in transport function. Exp Cell Res. 2002 Dec 10;281(2):205-12.
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