GlowDot40 Protein-Based Fluorescent Nanoparticles
These protein-based fluorescent nanoparticles are readily taken up by cells, biocompatible, and offer a variety of wavelengths to choose from multi-channel live cell imaging.
Highlights:
- Protein-Based - Low toxicity, biocompatible, and completely biodegradable
- Rapidly Internalized - Ideal for cell imaging
- Available in a variety of Excitation/Emission wavelengths
- Tested Cell Lines: HeLa, A549, HN12 (others not tested)
From the laboratory of C. Vijaya Kumar, PhD, University of Connecticut.
These protein-based fluorescent nanoparticles are readily taken up by cells, biocompatible, and offer a variety of wavelengths to choose from multi-channel live cell imaging.
Highlights:
- Protein-Based - Low toxicity, biocompatible, and completely biodegradable
- Rapidly Internalized - Ideal for cell imaging
- Available in a variety of Excitation/Emission wavelengths
- Tested Cell Lines: HeLa, A549, HN12 (others not tested)
From the laboratory of C. Vijaya Kumar, PhD, University of Connecticut.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Small Molecule |
| Internal Contents: | bovine serum albumin, organic dye, natural amino acids |
| Particle Morphology: | Sphere, rough, porous |
| Format: | 3mg in glucose matrix per tube |
| Spectral Information: |
GlowDot40-254W - Ex: 254nm; Em: 400, 520, 603nm GlowDot40-340 - Ex: 340nm; Em: 376nm GlowDot40-350 - Ex: 350nm; Em: 400nm GlowDot40-365W - Ex: 365nm; Em: 400, 520, 603nm GlowDot40-402W - Ex: 402nm; Em: 470, 520, 603nm GlowDot40-432 - Ex: 432nm; Em: 472nm GlowDot40-494 - Ex: 494nm; Em: 520nm GlowDot40-543 - Ex: 543nm; Em: 571nm GlowDot40-576 - Ex: 576nm; Em: 603nm |
| Size Distribution: | ±5nm |
| Mean Diameter: | 35nm |
| Surface Functionalization: | acid, base, hydroxyl, and others |
| Nanoparticle Consistency: | Uniform |
| Storage: | 4C |
| Shipped: | Cold packs |
Provider
From the laboratory of C. Vijaya Kumar, PhD, University of Connecticut.
Comments
Recommended Protocol
- Remove centrifuge tube from packaging.
- Re-suspend centrifuge tube contents in 10 mM Phosphate buffer pH 7.2 so that the final volume is 300 uL. This will yield a 10 mg/mL solution of GlowDots.
- Filter re-suspended GlowDots using a 13 mm diameter PVDF filter with 0.22 µm pore size.
- Incubate cells with 0.3 mg/mL for 1 hour.
- Image cells on confocal microscope with appropriate excitation laser.
References
- Deshapriya IK, Stromer BS, Pattammattel A, Kim CS, Iglesias-Bartolome R, Gonzalez-Fajardo L, Patel V, Gutkind JS, Lu X, Kumar CV. Fluorescent, bioactive protein nanoparticles (prodots) for rapid, improved cellular uptake. Bioconjug Chem. 2015 Mar 18;26(3):396-404.
If you publish research with this product, please let us know so we can cite your paper.
