GlowDot Protein-Based Fluorescent Nanoparticles

These protein-based fluorescent nanoparticles are readily taken up by cells, biocompatible, and offer a variety of wavelengths to choose from multi-channel live cell imaging.

Highlights:

  • Protein-Based - Low toxicity, biocompatible, and completely biodegradable
  • Rapidly Internalized - Ideal for cell imaging
  • Available in a variety of Excitation/Emission wavelengths
  • Tested Cell Lines: HeLa, A549, HN12 (others not tested)

From the laboratory of C. Vijaya Kumar, PhD, University of Connecticut.

Catalog Number Product Size AVAILABILITY Price Qty
ECN003
GlowDot 254W, 1mg
1mg In stock
Price: $103.00
ECN004
GlowDot 340, 1mg
1mg In stock
Price: $103.00
ECN005
GlowDot 350, 1mg
1mg In stock
Price: $103.00
ECN006
GlowDot 365W, 1mg
1mg In stock
Price: $103.00
ECN007
GlowDot 402W, 1mg
1mg In stock
Price: $103.00
ECN008
GlowDot 432, 1mg
1mg In stock
Price: $103.00
ECN009
GlowDot 494, 1mg
1mg In stock
Price: $103.00
ECN010
GlowDot 543, 1mg
1mg In stock
Price: $103.00
ECN011
GlowDot 576, 1mg
1mg In stock
Price: $103.00
Specifications
Product Type: Small Molecule
Name: 254W
Internal Contents: bovine serum albumin, organic dye, natural amino acids
Functionalization: acid, base, hydroxyl, and others
Particle Morphology: Sphere, rough, porous
Format: 1mg in glucose matrix per tube
Spectral Information: Ex: 254 nmEm: 400, 520, 603 nm
Size Distribution: ± 5 nm
Mean Diameter: 35 nm
Nanoparticle Consistency: Uniform
Storage: 4C
Shipped: Cold packs
Documentation
Product: 254W 340 350 365W 402W 432 494 543 576
Nanoparticle Consistency: Uniform Uniform Uniform Uniform Uniform Uniform Uniform Uniform Uniform
Particle Morphology: Sphere, rough, porous Sphere, rough, porous Sphere, rough, porous Sphere, rough, porous Sphere, rough, porous Sphere, rough, porous Sphere, rough, porous Sphere, rough, porous Sphere, rough, porous
Mean Diameter:

35 nm

35 nm

35 nm

35 nm

35 nm

35 nm

35 nm

35 nm

35 nm

Size Distribution: ± 5 nm ± 5 nm ± 5 nm ± 5 nm ± 5 nm ± 5 nm ± 5 nm ± 5 nm ± 5 nm
Functionalization: acid, base, hydroxyl, and others
Internal Contents: bovine serum albumin, organic dye, natural amino acids
Spectral Information: Ex: 254 nm
Em: 400, 520, 603 nm
Ex: 340 nm
Em: 376 nm
Ex: 350 nm
Em: 400 nm
Ex: 365 nm
Em: 400, 520, 603 nm
Ex: 402 nm
Em: 475, 520, 603nm
Ex: 432 nm
Em: 472 nm
Ex: 494 nm
Em: 520 nm
Ex: 543 nm
Em: 571 nm
Ex: 576 nm
Em: 603 nm
Form: 1mg in glucose matrix per tube
Storage Temperature: 4C 4C 4C 4C 4C 4C 4C 4C 4C
Shipped: Cold packs Cold packs Cold packs Cold packs Cold packs Cold packs Cold packs Cold packs Cold packs
Provider
From the laboratory of C. Vijaya Kumar, PhD, University of Connecticut.
Comments

Recommended Protocol

  1. Remove centrifuge tube from packaging.
  2. Re-suspend centrifuge tube contents in 10 mM Phosphate buffer pH 7.2 so that the final volume is 100 uL. This will yield a 10 mg/mL solution of GlowDots.
  3. Filter re-suspended GlowDots using a 13 mm diameter PVDF filter with 0.22 µm pore size.
  4. Incubate cells with 0.3 mg/mL for 1 hour.
  5. Image cells on confocal microscope with appropriate excitation laser.
References
  1. Deshapriya IK, Stromer BS, Pattammattel A, Kim CS, Iglesias-Bartolome R, Gonzalez-Fajardo L, Patel V, Gutkind JS, Lu X, Kumar CV. Fluorescent, bioactive protein nanoparticles (prodots) for rapid, improved cellular uptake. Bioconjug Chem. 2015 Mar 18;26(3):396-404.

If you publish research with this product, please let us know so we can cite your paper.

 
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