Marker-Free Transgenic Plant Vectors

Marker-Free Transgenic Plant Vectors allow for excision of a selection marker gene in primary transgenic plants by heat shock treatment and are suitable for transformation of plants with Agrobacterium tumefaciens.

Highlights:

  • pNS13: for cloning full gene (promoter:gene:terminator) into HindIII site
  • pNS14: for cloning cDNA into MluI sites (to be expressed by 35S promoter)
  • Binary Vector backbone: pPZP200 (spectinomycin selection)
  • Plant selection: NPT II gene (Kanamycin or Geneticin)
  • Excision system: Cre-lox recombination
  • Autoexcision system: Heat-inducible Cre-lox recombination

From the laboratory of Vibha Srivastava, PhD, University of Arkansas, Division of Agriculture.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EAK001
pNS13 Plant Transformation Vector, 5ug
5ug In stock
Price: $70.00
EAK002
pNS14 Plant Transformation Vector, 5ug
5ug In stock
Price: $70.00
Specifications
Product Type: Plasmid
Gene/insert name: pNS13, pNS14
Accession ID: HSP promoter (soybean hsp 17.5E): M28070
Antibiotic Resistance: pPZP200 (spectinomycin selection); NPT II gene (Kanamycin or Geneticin)
Format: Liquid (Domestic shipments), Spotted Filter Paper (International shipments)
Vector Backbone and Size: pPZP200; 10.6kb (pNS13), 11.3kb (pNS14)
Storage: Long term -20C
Shipped: Ambient temperature
Research

Plant Transformation Vectors

Between the loxP sites there are two fragments: (1) XbaI fragment containing 35S:NPT:nos3', (2) SacI fragment containing HSP:Cre:nos3'. Outside is a HindIII 35S::nos3' fragment with a unique cloning site, MluI.

Provider
From the laboratory of Vibha Srivastava, PhD, University of Arkansas, Division of Agriculture.
References
  1. Nandy S, Srivastava V. Marker-free site-specific gene integration in rice based on the use of two recombination systems. Plant Biotechnol J. 2012 Oct;10(8):904-12.

If you publish research with this product, please let us know so we can cite your paper.

 
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