Photoactivatable Serotonin (BHQ-O-5HT)
BHQ-O-5HT is a photoactivatable form of the neurotransmitter serotonin (5-HT) that is activated with either 1-photon excitation (1PE) at 368 nm or 2-photon excitation (2PE) at 740 nm in a biological preparation, such as cell culture, tissue slice, ex vivo brain, or whole animals.
Highlights:
- Has been used in dorsal root ganglia cell culture, ex vivo zebrafish brain, and whole animals (zebrafish and Xenopus laevis)
- Stable in pH 7.2 KMOPS buffer in the dark at room temperature for several days
Serotonin (5-HT) is a neuromodulator involved in regulating mood, appetite, memory, learning, pain, and establishment of left-right (LR) asymmetry in embryonic development. BHQ-O-5HT is a "caged" 5-HT molecule, that, upon photoactivation, releases 5-HT, enabling temporal control of the release of 5-HT within a biological system. BHQ-O-5HT depolarized sensory neurons when photolyzed in culture and in larval zebrafish comparable to that observed by 5-HT by itself. The quantum efficiency (Qu) of BHQ-O-5HT·2HBr for 1-photon photolysis is 0.40 at 365 nm in KMOPS buffer at pH 7.2. The 2-photon uncaging action cross-section (du) is 0.50 GM.
From the laboratories of Timothy M. Dore, PhD and James D. Lauderdale, PhD, University of Georgia.
Part of The Investigator's Annexe program.
BHQ-O-5HT is a photoactivatable form of the neurotransmitter serotonin (5-HT) that is activated with either 1-photon excitation (1PE) at 368 nm or 2-photon excitation (2PE) at 740 nm in a biological preparation, such as cell culture, tissue slice, ex vivo brain, or whole animals.
Highlights:
- Has been used in dorsal root ganglia cell culture, ex vivo zebrafish brain, and whole animals (zebrafish and Xenopus laevis)
- Stable in pH 7.2 KMOPS buffer in the dark at room temperature for several days
Serotonin (5-HT) is a neuromodulator involved in regulating mood, appetite, memory, learning, pain, and establishment of left-right (LR) asymmetry in embryonic development. BHQ-O-5HT is a "caged" 5-HT molecule, that, upon photoactivation, releases 5-HT, enabling temporal control of the release of 5-HT within a biological system. BHQ-O-5HT depolarized sensory neurons when photolyzed in culture and in larval zebrafish comparable to that observed by 5-HT by itself. The quantum efficiency (Qu) of BHQ-O-5HT·2HBr for 1-photon photolysis is 0.40 at 365 nm in KMOPS buffer at pH 7.2. The 2-photon uncaging action cross-section (du) is 0.50 GM.
From the laboratories of Timothy M. Dore, PhD and James D. Lauderdale, PhD, University of Georgia.
Part of The Investigator's Annexe program.
| Product Type: | Small Molecule |
| Name: | BHQ-O-5HT hydrogen bromide |
| Chemical Formula: | C20H20Br3N3O2 |
| Molecular Weight: | 574.11 |
| Format: | Orange solid |
| Purity: | >95%, HPLC |
| Stability: | >6 months at -20C |
| Solubility: | >5mg in 100 mL KMOPS buffer (pH 7.2) |
| Spectral Information: | λmax= 371nm; ε= 4700 M-1cm-1, Qu= 0.4 |
| Storage: | -20C; Protect from light |
| Shipped: | Dry ice |
Provided in salt form is for increased dry storage stability and easier handling.
Recommended dilution is 100uM at physiological pH. For best results, prepare solutions immediately prior to use. A stock solution in DMSO stored at -20C and in the dark has a reasonable shelf life. Once in aqueous enviornment, protect from light. Work under red light while handling the compounds before and during use.
- Rea AC, Vandenberg LN, Ball RE, Snouffer AA, Hudson AG, Zhu Y, McLain DE, Johnston LL, Lauderdale JD, Levin M, Dore TM. Light-activated serotonin for exploring its action in biological systems. Chem Biol. 2013 Dec 19;20(12):1536-46. doi: 10.1016/j.chembiol.2013.11.005.
- Vandenberg LN, Blackiston DJ, Rea AC, Dore TM, Levin M. Left-right patterning in Xenopus conjoined twin embryos requires serotonin signaling and gap junctions. Int J Dev Biol. 2014;58(10-12):799-809. doi: 10.1387/ijdb.140215ml.
- PCT Publication number WO2013003547A - University of Georgia Research Foundation, Inc. (Publ Jan 03, 2013).
If you publish research with this product, please let us know so we can cite your paper.
