NIH 2/4 Cell Line
The NIH 2/4 Cell Line allows for the direct study of the dynamics of single double-strand breaks (DSBs) and translocations in living mammalian cells in vivo.
NIH3T3 cells containing a copy of the unique 18 nucleotide ISceI restriction site flanked by an array of 256 copies of the lac-repressor binding site and by 96 copies of the tetracycline response element (LacOISceITetO array). This cell line does not express fluorescent repressors.
From the laboratory of Tom Misteli, PhD, National Cancer Institute/NIH.
Part of The Investigator's Annexe program.
The NIH 2/4 Cell Line allows for the direct study of the dynamics of single double-strand breaks (DSBs) and translocations in living mammalian cells in vivo.
NIH3T3 cells containing a copy of the unique 18 nucleotide ISceI restriction site flanked by an array of 256 copies of the lac-repressor binding site and by 96 copies of the tetracycline response element (LacOISceITetO array). This cell line does not express fluorescent repressors.
From the laboratory of Tom Misteli, PhD, National Cancer Institute/NIH.
Part of The Investigator's Annexe program.
This product is for sale to Nonprofit customers only. For profit customers, please Contact Us for more information.
| Product Type: | Cell Line |
| Name: | NIH 2/4 |
| Cell Type: | NIH3T3 mouse embryonic fibroblasts |
| Accession ID: | CVCL_5I45 |
| Organism: | Mouse |
| Source: | mouse embryo (NIH3T3 cells) |
| Morphology: | Fibroblast |
| Biosafety Level: | BSL1 |
| Subculturing: | Split 1 to 3 when ?90% confluency |
| Growth Conditions: | High-glucose DMEM, 10-15% FBS, P/S, Glutamine, 400 ug/ml hygromycin |
| Cryopreservation: | 15% DMSO in FBS |
| Storage: | Liquid nitrogen |
| Shipped: | Dry ice |
- Soutoglou E, Dorn JF, Sengupta K, Jasin M, Nussenzweig A, Ried T, Danuser G, Misteli T. Positional stability of single double-strand breaks in mammalian cells. Nat Cell Biol. 2007 Jun;9(6):675-82. Epub 2007 May 7.
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