Anti-CD20, C-Terminal [1440] Antibody

This rabbit polyclonal antibody [1440] was generated against a synthetic peptide corresponding to residues 231-245 (SAEEKKEQTIEIKEE) of human CD20 fused to ovalbumin, and is specific for human CD20 (intracellular C-terminal region).

Highlights:

  • Reacts with intracellular residues 231-245 of human CD20
  • Valuable for both pre-clinical and clinical research applications
  • Suitable for Western Blot and Immunoprecipitation applications

CD20, or B-lymphocyte antigen CD20, is a cell-surface protein expressed in most immune cells arising from the B-cell lineage and plays a role in cellular activation and proliferation. Importantly, CD20 is a recognized biomarker for a number of human disorders, including several autoimmune diseases and B-cell malignancies.

From the laboratory of Julie P. Deans, PhD, University of Calgary.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EUC005
Anti-CD20, C-Terminal [1440] Antibody, 100uL
100uL In stock
Price: $299.00
Specifications
Product Type: Antibody
Antigen: CD20 (CD20-C)
Molecular Weight: 33-35 kDa
Clonality: Polyclonal
Clone Name: 1440
Reactivity: Human
Immunogen: Peptide conjugated to ovalbumin
Species Immunized: Rabbit
Epitope: C-terminal residues 231-245; SAEEKKEQTIEIKEE
Buffer: Serum
Tested Applications: WB (1:2,000)
Storage: -80C
Shipped: Dry ice
Data

Western Blot of CD20 N and C-terminus

Western blots of human Ramos B-cells lysates probed with rabbit anti-CD20N (targeting the N-terminal residues 25-41) and rabbit anti-CD20C (targeting the C-terminal residues 231-245), pre-incubated with immunizing peptide (lanes 2 and 5), irrelevant peptide (lanes 3 and 6) or no peptide (lanes 1 and 4).

Adapted from: Polyak MJ, et al. J Immunol. 1998 Oct 1;161(7):3242-8.

Provider
From the laboratory of Julie P. Deans, PhD, University of Calgary.
References
  1. Polyak MJ, Tailor SH, Deans JP. Identification of a cytoplasmic region of CD20 required for its redistribution to a detergent-insoluble membrane compartment. J Immunol. 1998 Oct 1;161(7):3242-8.
  2. Deans JP, Robbins SM, Polyak MJ, Savage JA. Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment. J Biol Chem. 1998 Jan 2;273(1):344-8.
  3. Popoff IJ, Savage JA, Blake J, Johnson P, Deans JP. The association between CD20 and Src-family Tyrosine kinases requires an additional factor. Mol Immunol. 1998 Mar;35(4):207-14.
  4. Polyak MJ, Deans JP. Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure. Blood. 2002 May 1;99(9):3256-62.
  5. Polyak MJ, Li H, Shariat N, Deans JP. CD20 homo-oligomers physically associate with the B cell antigen receptor. Dissociation upon receptor engagement and recruitment of phosphoproteins and calmodulin-binding proteins. J Biol Chem. 2008 Jul 4;283(27):18545-52.

If you publish research with this product, please let us know so we can cite your paper.

 
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