Anti-Proinsulin Antibodies
This range of mouse mononclonal antibodies recognize feline proinsulin and are used to study feline metabolism and beta cell function.
Proinsulin is the precursor to the prohormone, insulin, as well as the protein, C-peptide made from beta cells in the pancreas. Mature insulin has 35 fewer amino acids than proinsulin, 4 are removed altogether and the remaining 31 form the C-peptide. The C-peptide is abstracted from the center of the proinsulin sequence and the two other ends (the B chain and A chain) remain connected by disulfide bonds.
From the laboratory of Margarethe E. Hoenig, PhD, University of Georgia.
Part of The Investigator's Annexe program.
This range of mouse mononclonal antibodies recognize feline proinsulin and are used to study feline metabolism and beta cell function.
Proinsulin is the precursor to the prohormone, insulin, as well as the protein, C-peptide made from beta cells in the pancreas. Mature insulin has 35 fewer amino acids than proinsulin, 4 are removed altogether and the remaining 31 form the C-peptide. The C-peptide is abstracted from the center of the proinsulin sequence and the two other ends (the B chain and A chain) remain connected by disulfide bonds.
From the laboratory of Margarethe E. Hoenig, PhD, University of Georgia.
Part of The Investigator's Annexe program.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Antibody |
| Antigen: | Anti-Proinsulin Antibodies |
| Accession ID: | Q52PU3 |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | 11E6.A5 |
| Reactivity: | Cat |
| Immunogen: | Protein |
| Species Immunized: | Mouse |
| Epitope: | Pro-insulin (possibly B chain) |
| Purification Method: | Protein G |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: | IRMA, ELISA |
| Storage: | -20C |
| Shipped: | Cold packs |
Anti-Proinsulin Antibodies
| Clone Name: | 11E6.A5 | 4H2.A8 | 7H3.A2 | 2E4.C2 | 4E11.A2.C3 | 8E7.B12 | 4F12.F2 | 6G3.F1 | 3C11.F1 |
| Antigen Name: | Anti-Proinsulin Antibodies | ||||||||
| Acession ID: | Q52PU3 (cat preproinsulin) | ||||||||
| Immunogen Isotype: | IgG1 | IgG2b | IgG1 | IgG1 | IgG1 | IgG2b | IgG1 | IgG1 | IgG1 |
| Clonality: | Monoclonal | ||||||||
| Species Reactivity: | Cat | Cat | Cat | Cat | Cat | Cat | Cat | Cat | Cat |
| Epitope: | Pro-insulin (possibly B chain) | Pro-insulin | Pro-insulin (possibly B chain) | Pro-insulin (PCI) | Pro-insulin (B chain) | Pro-insulin (PC1 and PC2) | Pro-insulin (PC1) | Pro-insulin (C peptide) | Pro-insulin |
| Species Immunized: | Mouse | ||||||||
| Immunogen: | Protein | ||||||||
| Purification Method: | Protein G | ||||||||
| Storage Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide | ||||||||
| Tested Applications: | IRMA, ELISA | ELISA | ELISA | ELISA | ELISA | IRMA, ELISA | ELISA | ELISA | ELISA |
| Storage Temperature: | -20C | ||||||||
| Shipped: | Cold packs | ||||||||
Estimated Location of Epitopes for Antibodies
Possible location of epitopes recognized by each antibody.
Adapted from: Caffall. ZF. The University of Georgia, Athens, GA (2006).
Clone 4H2.A8 is complementary to clones 11E6.A5 and 8E7.B12.
- Caffall, ZF. Development of monoclonal and polyclonal antibodies against feline proinsulin and C-peptide. MS Thesis. The University of Georgia, Athens, GA (2006).
- Kley S, Caffall Z, Tittle E, Ferguson DC, Hoenig M. Development of a feline proinsulin enzyme-linked immunosorbent assay (ELISA): A novel application to examine beta cell function in cats. Domest Anim Endocrinol. 2008 Apr;34(3):311-8.
- Hoenig M, Traas AM, Schaeffer DJ. Evolution of routine hematology profile results and fructosamine, tyroxine, insulin, and proinsulin concentrations in lean, overweight, obese and diabetic cats. J Am Vet Med Assoc. 2013 Nov 1;243(9):1302-9.
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