Anti-CD4 [8A5] Antibody

This mouse IgM monoclonal antibody recognizes the amino-acid sequence corresponding to the 3'-UGA codon region of human cluster of differentiation antignen 4 (CD4).

Highlights:

  • Reacts with the region of the human CD4 gene downstream of the natural UGA (STOP) codon transcribed, if termination at the UGA codon is suppressed
  • Ideal for studies of the role of selenium metabolism and selenoproteins in diseases, such as infectious diseases and cancer
  • Tested for Western Blot, Immunofluorescence, and ELISA applications

Eukaryotic genes end with one of three stop codons, UGA, UAA, or UAG. Of these three, UGA is unique. In addition to functioning as a stop codon, UGA is also the codon for the twenty-first amino acid, selenocysteine. The biological bases for the dual function of the UGA codon are not entirely clear. Selenocysteine plays a critical role in physiology, and perturbations of selenium intake and metabolism can have disastrous consequences. CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus.

From the laboratory of Ethan W. Taylor, PhD, University of Georgia.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EGA901
Anti-CD4 [8A5] Antibody (supernatant), 15mL
Supernatant, 15mL 2-3 weeks
Price: $285.00
Specifications
Product Type: Antibody
Antigen: RFLPADQMNVADPQPLASCS of human CD4
Accession ID: P01730
Isotype: IgMk
Clonality: Monoclonal
Clone Name: 8A5, also known as Antibody 025
Reactivity: Human (possibly others)
Immunogen: Peptide
Species Immunized: Mouse
Purification Method: Not purified
Buffer: Cell culture supernatant
Tested Applications: Use undiluted or up to 1:300. Dilute in PBS or other compatible medium as required by specific experiment. For ELISA: 10-25 ug/ml (Jurkat, H9, HeLa). For IF: 5-12 ug/ml
Storage: -80C (long term)
Shipped: Dry ice
Provider
From the laboratory of Ethan W. Taylor, PhD, University of Georgia.
References
  1. Ware, B.N.: Gene regulation and overlooked coding regions : the case for translation past the 3'-UGA codon in CD4. Ph.D. Thesis. University of Georgia, College of Pharmacy.(2003)

If you publish research with this product, please let us know so we can cite your paper.

 
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