Anti-eEF1A Antibody
This rabbit polyclonal antibody was generated against full length protein and is specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 1A (eEF1A).
Highlights
- Specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 1A (eEF1A) - Does NOT recognize human eEF1A
- Suitable for Western Blot applications
Elongation factors are proteins used in translation or protein synthesis during the process of cell cycle and elongation in some cells. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. Specifically, elongation factor 1 alpha is the GTP-binding component of the eukaryotic elongation factor 1 complex (eEF1). In its active GTP-bound form, it binds to and delivers aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. The inactive GDP-bound form leaves the ribosome and must be recycled by its guanine nucleotide exchange factor (GEF) (eEF1B subcomplex) before binding another molecule of aminoacyl-tRNA. eEF1A is required for nuclear export of aminoacyl-tRNAs, and may also be involved in translational quality control by targeting cotranslationally damaged proteins to the proteasome. eEF1A also exhibits actin filament-binding and -bundling activities and is involved in cytoskeleton organization.
From the laboratory of Terri Goss Kinzy, PhD, Rutgers University.
This rabbit polyclonal antibody was generated against full length protein and is specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 1A (eEF1A).
Highlights
- Specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 1A (eEF1A) - Does NOT recognize human eEF1A
- Suitable for Western Blot applications
Elongation factors are proteins used in translation or protein synthesis during the process of cell cycle and elongation in some cells. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. Specifically, elongation factor 1 alpha is the GTP-binding component of the eukaryotic elongation factor 1 complex (eEF1). In its active GTP-bound form, it binds to and delivers aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. The inactive GDP-bound form leaves the ribosome and must be recycled by its guanine nucleotide exchange factor (GEF) (eEF1B subcomplex) before binding another molecule of aminoacyl-tRNA. eEF1A is required for nuclear export of aminoacyl-tRNAs, and may also be involved in translational quality control by targeting cotranslationally damaged proteins to the proteasome. eEF1A also exhibits actin filament-binding and -bundling activities and is involved in cytoskeleton organization.
From the laboratory of Terri Goss Kinzy, PhD, Rutgers University.
| Product Type: | Antibody |
| Antigen: | Saccharomyces cerevisiaeeukaryotic Translation Elongation Factor 1A (eEF1A) |
| Accession ID: | P68104, NP_015405 |
| Molecular Weight: | 50 kDa |
| Clonality: | Polyclonal |
| Reactivity: | Saccharomyces cerevisiae, other fungi |
| Immunogen: | Full length protein |
| Species Immunized: | Rabbit |
| Buffer: | Lyophilized whole serum |
| Tested Applications: | WB (1:10,000) |
| Storage: | 4C |
| Shipped: | Ambient temperature |
Protocol Notes
Reconstitute in 25uL water.
NotesDoes not recognize human eEF1A.
- Carr-Schmid A, Valente L, Loik VI, Williams T, Starita LM, Kinzy TG. Mutations in elongation factor 1beta, a guanine nucleotide exchange factor, enhance translational fidelity. Mol Cell Biol. 1999 Aug;19(8):5257-66.
- Jindal S, Ghosh A, Ismail A, Singh N, Komar AA. Role of the uS9/yS16 C-terminal tail in translation initiation and elongation in Saccharomyces cerevisiae. Nucleic Acids Res. 2018 Nov 27. View Article
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