Human Pancreatic Duct Epithelial Cell Line (H6c7)

H6c7 is an immortalized epithelial cell line derived from normal human pancreatic duct epithelial (HPDE) cells. This cell line is an attractive model for human pancreatic duct cell carcinogenesis studies.

Highlights

  • Demonstrate near normal genotype and phenotype of HPDE cells
  • Display copy number of p16INK4A gene, wild type p53 and KRAS genes
  • Morphologically similar to the primary HPDE cells, grow anchorage-dependently and do not form tumors in scid mice
  • Growth remains inhibited by transforming growth factor (TGF)-beta 1

From the laboratory of Ming-Sound Tsao, MD, FRCPC, University Health Network, Canada.

Catalog Number Product Size AVAILABILITY Price Qty
ECA001-FP
Human Pancreatic Duct Epithelial Cell Line (H6c7), 1 Vial (Non-Profit)
1 vial Limited Stock
Price: $799.00

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Specifications
Product Type: Cell Line
Name: H6C7
Cell Type: Epithelial
Accession ID: CVCL_0P38
Organism: Human
Source: Pancreatic duct
Morphology: Epithelial
Biosafety Level: BSL2
Subculturing: 1:4 split
Growth Conditions: Keratinocyte serum free
Cryopreservation: Growth medium plus 10% DMSO
Mycoplasma Tested: Yes
Storage: Liquid nitrogen
Shipped: Dry ice
Documentation
Provider
From the laboratory of Ming-Sound Tsao, MD, FRCPC, University Health Network, Canada.
References
  1. Radulovich N, Qian JY, Tsao MS. Human pancreatic duct epithelial cell model for KRAS transformation. Methods Enzymol. 2008;439:1-13.
  2. Qian J, Niu J, Li M, Chiao PJ, Tsao MS. In vitro modeling of human pancreatic duct epithelial cell transformation defines gene expression changes induced by K-ras oncogenic activation in pancreatic carcinogenesis. Cancer Res. 2005 Jun 15;65(12):5045-53.
  3. Furukawa T, Duguid WP, Rosenberg L, Viallet J, Galloway DA, Tsao MS. Long-term culture and immortalization of epithelial cells from normal adult human pancreatic ducts transfected by the E6E7 gene of human papilloma virus 16. Am J Pathol. 1996 Jun;148(6):1763-70.
  4. Hu J, Sheng Y, Kwak KJ, Shi J, Yu B, Lee LJ. A signal-amplifiable biochip quantifies extracellular vesicle-associated RNAs for early cancer detection. Nat Commun. 2017 Nov 22;8(1):1683. View Article
  5. Gilles ME, Hao L, Huang L, Rupaimoole R, Lopez-Casas PP, Pulver E, Jeong JC, Muthuswamy SK, Hidalgo M, Bhatia SN, Slack FJ. Personalized RNA Medicine for Pancreatic Cancer. Clin Cancer Res. 2018 Apr 1;24(7):1734-1747. View Article
  6. Wei W, Liu Y, Lu Y, Yang B, Tang L. LncRNA XIST Promotes Pancreatic Cancer Proliferation Through miR-133a/EGFR. J Cell Biochem. 2017 Oct;118(10):3349-3358. View Article
  7. Tanton H, Voronina S, Evans A, Armstrong J, Sutton R, Criddle DN, Haynes L, Schmid MC, Campbell F, Costello E, Tepikin AV. F(1)F(0)-ATP Synthase Inhibitory Factor 1 in the Normal Pancreas and in Pancreatic Ductal Adenocarcinoma: Effects on Bioenergetics, Invasion and Proliferation. Front Physiol. 2018 Jul 11;9:833. View Article
  8. Zhu G, Zhou L, Liu H, Shan Y, Zhang X. MicroRNA-224 Promotes Pancreatic Cancer Cell Proliferation and Migration by Targeting the TXNIP-Mediated HIF1α Pathway. Cell Physiol Biochem. 2018 Aug 3;48(4):1735-1746. View Article
  9. Liu B, Palmfeldt J, Lin L, Colaço A, Clemmensen KKB, Huang J, Xu F, Liu X, Maeda K, Luo Y, Jäättelä M. STAT3 associates with vacuolar H(+)-ATPase and regulates cytosolic and lysosomal pH. Cell Res. 2018 Aug 20. View Article
  10. Park JY, Cho YL, Chae JR, Moon SH, Cho WG, Choi YJ, Lee SJ, Kang WJ. Gemcitabine-Incorporated G-Quadruplex Aptamer for Targeted Drug Delivery into Pancreas Cancer. Mol Ther Nucleic Acids. 2018 Sep 7;12:543-553. View Article
  11. Thomas NA, Abraham RG, Dedi B, Krucher NA. Targeting retinoblastoma protein phosphorylation in combination with EGFR inhibition in pancreatic cancer cells. Int J Oncol. 2018 Dec 7. View Article
  12. Lee J, Lee J, Choi C, Kim JH. Blockade of integrin α3 attenuates human pancreatic cancer via inhibition of EGFR signalling. Sci Rep. 2019 Feb 26;9(1):2793. View Article
  13. Lee J, Lee J, Kim JH. Scattered DUSP28 is a novel biomarker responsible for aggravating malignancy via the autocrine and paracrine signaling in metastatic pancreatic cancer. Cancer Lett. 2019 Aug 1;456:1-12. View Article
  14. Stefanius K, Servage K, de Souza Santos M, Gray HF, Toombs JE, Chimalapati S, Kim MS, Malladi VS, Brekken R, Orth K. Human pancreatic cancer cell exosomes, but not human normal cell exosomes, act as an initiator in cell transformation. Elife. 2019 May 28;8. pii: e40226. View Article
  15. Lutz MM 4th, Worth MP, Hinchman MM, Parker JSL, Ledgerwood ED. Mammalian orthoreovirus Infection is Enhanced in Cells Pre-Treated with Sodium Arsenite. Viruses. 2019 Jun 18;11(6). pii: E563. View Article
  16. Yu Y, Liang S, Zhou Y, Li S, Li Y, Liao W. HNF1A/CASC2 regulates pancreatic cancer cell proliferation through PTEN/Akt signaling. J Cell Biochem. 2019 Mar;120(3):2816-2827. View Article
  17. Gilles ME, Hao L, Huang L, Rupaimoole R, Lopez-Casas PP, Pulver E, Jeong JC, Muthuswamy SK, Hidalgo M, Bhatia SN, Slack FJ. Personalized RNA Medicine for Pancreatic Cancer. Clin Cancer Res. 2018 Apr 1;24(7):1734-1747. View Article
  18. Heining C, Horak P, Uhrig S, Codo PL, Klink B, Hutter B, Fröhlich M, Bonekamp D, Richter D, Steiger K, Penzel R, Endris V, Ehrenberg KR, Frank S, Kleinheinz K, Toprak UH, Schlesner M, Mandal R, Schulz L, Lambertz H, Fetscher S, Bitzer M, Malek NP, Horger M, Giese NA, Strobel O, Hackert T, Springfeld C, Feuerbach L, Bergmann F, Schröck E, von Kalle C, Weichert W, Scholl C, Ball CR, Stenzinger A, Brors B, Fröhling S, Glimm H. NRG1 Fusions in KRAS Wild-Type Pancreatic Cancer. Cancer Discov. 2018 Sep;8(9):1087-1095. View Article
  19. Gao M, Lin M, Moffitt RA, Salazar MA, Park J, Vacirca J, Huang C, Shroyer KR, Choi M, Georgakis GV, Sasson AR, Talamini MA, Kim J. Direct therapeutic targeting of immune checkpoint PD-1 in pancreatic cancer. Br J Cancer. 2019 Jan;120(1):88-96. View Article
  20. Zhou WY, Zhang MM, Liu C, Kang Y, Wang JO, Yang XH. Long noncoding RNA LINC00473 drives the progression of pancreatic cancer via upregulating programmed death-ligand 1 by sponging microRNA-195-5p. J Cell Physiol. 2019 Jun 17. View Article
  21. Silva C, Correia-Branco A, Andrade N, Ferreira AC, Soares ML, Sonveaux P, Stephenne J, Martel F. Selective pro-apoptotic and antimigratory effects of polyphenol complex catechin:lysine 1:2 in breast, pancreatic and colorectal cancer cell lines. Eur J Pharmacol. 2019 Jul 10;859:172533. View Article

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