TLR4 SNP Expressing Mouse Hepatic Stellate Cell Lines
These mouse hepatic stellate cell (HSC) lines express WT TLR4 or TLR4 single nucleotide polymorphisms (SNP) variants, D299G or T399I.
Hepatic stellate cells (also known as perisinusoidal cells or Ito cells) are the major cell type in the liver involved in fibrosis. Fibrosis is the first layer of scar tissue, when the liver becomes damaged. TLR4 or toll-like receptor 4, is a transmembrane protein that recognizes gram-negative bacteria and plays an important role in activating the innate immune system.
From the laboratory of Scott L. Friedman, MD, Icahn School of Medicine at Mount Sinai.
Part of The Investigator's Annexe program.
These mouse hepatic stellate cell (HSC) lines express WT TLR4 or TLR4 single nucleotide polymorphisms (SNP) variants, D299G or T399I.
Hepatic stellate cells (also known as perisinusoidal cells or Ito cells) are the major cell type in the liver involved in fibrosis. Fibrosis is the first layer of scar tissue, when the liver becomes damaged. TLR4 or toll-like receptor 4, is a transmembrane protein that recognizes gram-negative bacteria and plays an important role in activating the innate immune system.
From the laboratory of Scott L. Friedman, MD, Icahn School of Medicine at Mount Sinai.
Part of The Investigator's Annexe program.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Cell Line |
| Name: | TLR4-/- 299, TLR4-/- 399, TLR4-/- 299/399 |
| Cell Type: | Immortalized TLR4-/- mouse hepatic stellate cells |
| Accession ID: | O00206 |
| Organism: | Mouse transfected with human TLR4 D299G or T399I SNP cDNA |
| Source: | Liver |
| Morphology: | Hepatic stellate cells |
| Biosafety Level: | BL1 |
| Growth Conditions: | DMEM, 10% FBS |
| Cryopreservation: | Growth Medium + DMSO |
| Storage: | Liquid nitrogen |
| Shipped: | Dry ice |
NF-kB assay
Fold change of NF-kB reporter activity in TLR4-/- mouse HSCs transfected with TLR4 WT or SNP sequences, or a LacZ control vector, and in MyD88-/- HSCs reconstituted with WT TLR4 sequene. Cells expressing either the single or dual SNPs had less NF-kB activation.
Adapted from: Guo J., et al., Hepatology. 2009 Mar;49(3):960-8.
- Guo J, Loke J, Zheng F, Hong F, Yea S, Fukata M, Tarocchi M, Abar OT, Huang H, Sninsky JJ, Friedman SL. Functional linkage of cirrhosis-predictive single nucleotide polymorphisms of Toll-like receptor 4 to hepatic stellate cell responses. Hepatology. 2009 Mar;49(3):960-8.
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