Non-Natural Promoter T7 Polymerase
A modified T7 RNA polymerase that orthogonally recognizes a non-natural promoter sequence: 5’-TAATACCGGTCACTATA, where the underlined residues differ from wild-type.
Highlights:
- Does not recognize the wildtype promoter, nor does the wild type recognize the non natural promoter
From the laboratory of Andrew D. Ellington, PhD, University of Texas at Austin
Part of The Investigator's Annexe program.
A modified T7 RNA polymerase that orthogonally recognizes a non-natural promoter sequence: 5’-TAATACCGGTCACTATA, where the underlined residues differ from wild-type.
Highlights:
- Does not recognize the wildtype promoter, nor does the wild type recognize the non natural promoter
From the laboratory of Andrew D. Ellington, PhD, University of Texas at Austin
Part of The Investigator's Annexe program.
Specifications
| Product Type: | Protein |
| Name: | CGG-R12-KIR |
| Source: | Escherichia coli |
| Molecular Weight: | 100.142 kDa |
| Amino Acid Sequence: | MRGSHHHHHHGSNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVEDIPAIEREELPMKPEDIDMNPEALTAWKRAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAKKVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIKTRVHIMFLGQFEMQPTINTNKDSEIDARKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLNLRDILESDFAFA |
| Fusion Tag(s): | 6xHis |
| Purity: | > 98% (SDS-PAGE) |
| Buffer: | 50 mM Tris, 50 mM NaCl, 1 mM DTT, 0.1% Triton-X 100, 0.1 mM EDTA, 50% glycerol |
| Concentration: | 0.5mg/mL |
| Activity: | One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 ?l in 1 hour at 37°C in 1X RNA Polymerase Reaction Buffer |
| Comments: | Promoter specificity change (CGG) |
| Storage: | -20C |
| Shipped: | Cold packs |
Provider
From the laboratory of Andrew D. Ellington, PhD, University of Texas at Austin
References
- Ellefson JW, Meyer AJ, Hughes RA, Cannon JR, Brodbelt JS, Ellington AD. Directed evolution of genetic parts and circuits by compartmentalized partnered replication. Nat Biotechnol. 2014 Jan;32(1):97-101. doi: 10.1038/nbt.2714.
