2,5 dibromohexanediamide (DBHDA)
Alkylating agent that mildly converts cysteine to dehydroalanine (Dha) residues.
Highlights:
- Using a "tag-and-modify" approach, Dha can be used as a "handle" for further modifications
- Various nucleophiles (e.g., thiols, thiophosphate, etc.) can react with Dha to afford a wide range of chemical modifications
From the laboratory of Benjamin G. Davis, PhD, University of Oxford.
Part of The Investigator's Annexe program.
Alkylating agent that mildly converts cysteine to dehydroalanine (Dha) residues.
Highlights:
- Using a "tag-and-modify" approach, Dha can be used as a "handle" for further modifications
- Various nucleophiles (e.g., thiols, thiophosphate, etc.) can react with Dha to afford a wide range of chemical modifications
From the laboratory of Benjamin G. Davis, PhD, University of Oxford.
Part of The Investigator's Annexe program.
Specifications
| Product Type: | Small Molecule |
| Name: | 2,5 Dibromohexanediamide (DBHDA) |
| Chemical Formula: | C6H10Br2N2O2 |
| Molecular Weight: | 299.91 kDa |
| Format: | Off white solid |
| Purity: | > 95% by 1HNMR Purity Information |
| Solubility: | Soluble in DMSO and DMF, slightly soluble in methanol and H2O |
| Storage: | Stable at room temperature. Store at -20C for long term storage |
| Shipped: | Ambient temperature |
Documentation
Purity Information
Product Data sheet
Protocol Notes
- Typically 200 equivalents of DBHDA are added to a protein solution in buffer.
- Shake at 37C for 1-4 hours.
- Desalting provides a pure chemically modified protein.
Provider
From the laboratory of Benjamin G. Davis, PhD, University of Oxford.
References
- Chalker JM, Gunnoo SB, Boutureira O, Gerstberger SC, Fernández-González M, Bernardes GJL Griffin L, Hailu H, Schofielda CJ, Davis BG. Methods for converting cysteine to dehydroalanine on peptides and proteins. Chem. Sci., 2011,2, 1666-1676.
- Nadal S, Raj R, Mohammed S, Davis BG. Synthetic post-translational modification of histones. Curr Opin Chem Biol. 2018 Feb 28;45:35-47. View Article
- Dadová J, Galan SR, Davis BG. Synthesis of modified proteins via functionalization of dehydroalanine. Curr Opin Chem Biol. 2018 Jun 15;46:71-81. View Article
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