Endoglycosidase F1 (Endo F1)

Endoglycosidase F1 (Endo F1) from Elizabethkingia miricola, is an enzyme used for removing N-linked oligosaccharides from glycoproteins.

Endoglycosidase F1, expressed in E. coli, cleaves asparagine linked or free high mannose (oligomannose) and hybrid structures. It differs from exoglycosidase, that is does not do so at the terminal residue. This enzyme is suitable for deglycoslylation of N-linked oligosaccharides from glycoproteins under native conditions, and is appropriate for a variety of glycomics, proteomics, and mass spectroscopy applications.

From the laboratory of Davide Comoletti, DVM, PhD, Rutgers University

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
ED5002
Endoglycosidase F1 (Endo F1), 250ug
250ug Currently Unavailable
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Specifications
Product Type: Protein
Name: Endoglycosidase F1 (endo F)
Accession ID: Uniprot P36911
Source: Elizabethkingia miricola
Molecular Weight: 63 kDa (with the Tag)
Fusion Tag(s): GST
Buffer: 20 mM TRIS pH 8.0, 150 mM NaCl, 1 mM DTT, 20 % Glycerol
Concentration: 8mg/mL
Comments: Plasmid pGEX 3X, Expressed in E. coli BL21 (DE3)
Storage: -80C
Shipped: Dry ice
Documentation

Protocol Notes

For efficient deglycosylation, use overnight incubation (~16 h) at 4 deg C with a ratio 1:20 (ug enzyme:ug target protein), in mid pH conditions (Suggested; 50 mM TRIS pH 7.5, 150 mM NaCl).

Data

SDS PAGE

SDS-PAGE analysis showing purification of PNGase F on the left, Ladder in the middle, and Endo F1 on the right.

Provider
From the laboratory of Davide Comoletti, DVM, PhD, Rutgers University
References
  1. Ao J, Chinnici JL, Maddi A, Free SJ. The N-Linked Outer Chain Mannans and the Dfg5p and Dcw1p Endo-α-1,6-Mannanases Are Needed for Incorporation of Candida albicans Glycoproteins into the Cell Wall. Eukaryot Cell. 2015 Aug;14(8):792-803. View Article

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