pSD VSV-M (M51R) plasmid
Vesicular stomatitis virus (VSV) is a well studied, enveloped, negative-strand RNA virus. The VSV genome encodes for 5 proteins: N, P, M, G, and L. The M protein (or matrix protein) is responsible for binding the nucleocapsid and condenses it into a tightly coiled helix and binds the nucleocapsid to the envelope. This activity of the M protein is what gives the virus its bullet like shape. In addition to M protein's role in virus assembly, it is also responsible for mediating molecular mechanisms of VSV pathogenesis. Wild-type M protein surpresses host gene expression in infected cells and inhibits antiviral responses. This activity is lost when the methionine at position 51 is substituted with an argenine (M51R). This M protein mutant maintains its ability to function in VSV virion assembly, but is unable to surpress host gene expression. This in vitro expression vector encodes for M51R mutant M protein.
From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.
Part of The Investigator's Annexe program.
Vesicular stomatitis virus (VSV) is a well studied, enveloped, negative-strand RNA virus. The VSV genome encodes for 5 proteins: N, P, M, G, and L. The M protein (or matrix protein) is responsible for binding the nucleocapsid and condenses it into a tightly coiled helix and binds the nucleocapsid to the envelope. This activity of the M protein is what gives the virus its bullet like shape. In addition to M protein's role in virus assembly, it is also responsible for mediating molecular mechanisms of VSV pathogenesis. Wild-type M protein surpresses host gene expression in infected cells and inhibits antiviral responses. This activity is lost when the methionine at position 51 is substituted with an argenine (M51R). This M protein mutant maintains its ability to function in VSV virion assembly, but is unable to surpress host gene expression. This in vitro expression vector encodes for M51R mutant M protein.
From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.
Part of The Investigator's Annexe program.
| Product Type: | Plasmid |
| Gene/insert name: | VSV M-protein (M51R) |
| Alternative Name(s): | Matrix protein |
| Accession ID: | P03519 |
| Antibiotic Resistance: | Ampicilin |
| Fusion Tag(s): | poly(A-T) sequence (~85bp) |
| Grow in E. coli at 37 C: | yes |
| Insert Size: | ~800bp |
| Vector Backbone and Size: | pSD |
| High or low copy: | High copy |
| Shipped: | Ambient temperature, spotted on filter paper |
This plasmid is used to generate in vitro transcribed M-protein mRNA for transfection. The M-gene is under control of the sp6 bacteriophage promoter. A poly(A-T) sequence has been inserted after the M-gene. For in vitro transcription, linearize the plasmid with the restriction enzyme, SalI (downstream of poly(A-T) sequence.
- Black BL, Brewer B, Lyles DS. Effect of Vesicular Stomatitis Virus Matrix Protein onHost-Directed Translation In Vivo. J Virol. 1994; 68(1):550-560.
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