pSD VSV-M plasmid

Vesicular stomatitis virus (VSV) is a well studied, enveloped, negative-strand RNA virus. The VSV genome encodes for 5 proteins: N, P, M, G, and L. The M protein (or matrix protein) is responsible for binding the nucleocapsid and condenses it into a tightly coiled helix and binds the nucleocapsid to the envelope. This activity of the M protein is what gives the virus its bullet like shape. In addition to M protein's role in virus assembly, it is also responsible for mediating molecular mechanisms of VSV pathogenesis. Wild-type M protein surpresses host gene expression in infected cells and inhibits antiviral responses. This in vitro expression vector encodes for wild-type M protein .

From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EB0003
pSD VSV-M plasmid
Spotted on filter paper In stock
Regular Price:$115.00
On Sale:
Specifications

Product Type: Plasmid
Alternative Name(s): Matrix protein
Gene/insert name: VSV M-protein (rwt)
Accession ID: P03519
Antibiotic Resistance: Ampicilin
Fusion Tag(s): poly(A-T) sequence (~85bp)
Grow in E. coli at 37 C: yes
Insert Size: ~800bp
Vector Backbone and Size: pSD
High or low copy: High Copy
Shipped: Ambient temperature, spotted on filter paper

Provider
From the laboratory of Douglas S. Lyles, PhD, Wake Forest School of Medicine.
Comments

This plasmid is used to generate in vitro transcribed M-protein mRNA for transfection. The M-gene is under control of the sp6 bacteriophage promoter. A poly(A-T) sequence has been inserted after the M-gene. For in vitro transcription, linearize the plasmid with the restriction enzyme, SalI (downstream of poly(A-T) sequence.

References
  1. Black BL, Brewer B, Lyles DS. Effect of Vesicular Stomatitis Virus Matrix Protein onHost-Directed Translation In Vivo. J Virol. 1994; 68(1):550-560.

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