Anti-ADAM28 (mouse), Cytoplasmic Domain Antibody

This rabbit IgG polyclonal antibody was generated against the GST-ADAM28-cytoplasmic tail and is specific for the cytoplasmic domain of mouse ADAM28.

The ADAM (A Disintegrin And Metalloprotease Domain) family is a unique group of proteins that has the ability to cleave or shed extracellular portions of transmembrane proteins. In addition, ADAMs are involved in cell-cell and cell-matrix interactions (e.g., fertilization, muscle development and neurogenesis).

Based on sequence identity, ADAM28 is more closely related to snake venom metalloproteases (SVMPs) than to presently known ADAMs. Northern blot analysis shows that ADAM28 is expressed in the epididymis, suggesting a possible role in sperm maturation, and in the lung. Mature ADAM28 is presumed to be catalytically active and found mainly on the cell surface.

From the laboratory of Carl P. Blobel, MD, PhD, Hospital for Special Surgery.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EHS011
Anti-ADAM28 (mouse), Cytoplasmic Domain Antibody, 100uL
100uL In stock
Price: $299.00
Specifications
Product Type: Antibody
Accession ID: AF153350
Antigen: GST-ADAM28 Cytoplasmic Domain
Molecular Weight: ~100 kDa
Isotype: IgG
Clonality: Polyclonal
Reactivity: Mouse
Immunogen: GST-fusion protein with the cytoplasmic domain of mouse ADAM28
Species Immunized: Rabbit
Epitope: Cytoplasmic domain
Purification Method: Serum, not purified
Tested Applications: WB (1:500)
Storage: -80C
Shipped: Dry ice
Research
Antibody Species Reactivity Specificity
ADAM9 Human Cytoplasmic Domain
ADAM9 Mouse Cytoplasmic Domain
ADAM12 Mouse Cytoplasmic Domain
ADAM15 Human Cytoplasmic Domain
ADAM15 Mouse Cytoplasmic Domain
ADAM15 Human Extracellular Domain
ADAM15 Mouse Disintegrin Domain
ADAM17 Mouse Cytoplasmic Domain
ADAM17 Mouse Pro domain
ADAM19 Mouse Cytoplasmic Domain
ADAM28 Mouse Cytoplasmic Domain
ADAM28 Mouse Extracellular Domain
SNX9 Mouse Cytoplasmic Domain
Data

Western Blot Analysis

Western blot of lysates from COS-7 cells transfected with a pcDNA control and wild-type ADAM28 (WT). All samples were reduced with DTT and separated on a 7.5% (w/v) polyacrylamide gel. Proteins were transferred to nitrocellulose and immunoblotted with ADAM28 cytoplasmic domain antiserum.

Adapted from: Howard L, et al. Biochem J. 2000, 348, 21-27.

Provider
From the laboratory of Carl P. Blobel, MD, PhD, Hospital for Special Surgery.
References
  1. Howard, L.; Maciewicz R. A.; Blobel C. P. Cloning and characterization of ADAM28: evidence for autocatalytic pro-domain removal and for cell surface localization of mature ADAM28. Biochem J. 2000, 348, 21-27.

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